ESPE Abstracts (2014) 82 P-D-1-1-64

Biomarkers of Subclinical Inflammation in an Infant-Juvenile Population with Type 1 Diabetes

Maria Bazana, Sofia Casellaa, Santiago Lopeza, Teresita Carrizob, Maria Velardeb, Elba Díazb & Adela Abregub

aMedicine Faculty, National University of Tucuman, Tucuman, Argentina; bBiochemical Faculty, National University of Tucuman, Tucuman, Argentina

Background: Diabetes is associated with increased risk of vascular disease. In children and adolescents with type 1 diabetes (T1D), clinical manifestations of vascular complications are infrequent; however, a pro-inflammatory state and endothelial disturbance could appear early. A subclinical inflammation state result in increased plasma levels of adhesion molecules, inflammatory cytokines as tumor necrosis factor alpha (TNFα), and acute phase proteins as C-reactive protein (CRP) and fibrinogen (Fg).

Objective and hypotheses: The objective of this work was study plasma levels of soluble E-selectin (sE-S), VCAM1, TNFα, high sensitivity CRP (hsCRP) and Fg in a pediatric population with T1D, without clinical evidence of vascular complications; and the relationship with glycemic control and disease evolution.

Method: Forty-two T1D patients and 20 control children, age 8–13 years, were studied. Biochemical parameters evaluated were: white blood cell count (WBC), sE-S and VCAM1, TNFα, hsCPR, and plasma Fg. Retinopathy and nephropathy was discarded cart by ophthalmic exam and microalbuminuria determination respectively.

Results: In diabetic patients compared with the control group found increased levels of sE-S (108 (69–150) vs 68 (52–86) ng/ml, P=0.003), VCAM1 (785 (732–835) vs 712 (658–758) ng/ml, P=0.04), and hsPCR (1.00 (0.67–1.70) vs 0.20 (0.18–0.87) mg/l, P=0.01). No significant differences on WBC, TNFα, or Fg values between diabetics and controls were found. When diabetic patients were grouped according glycemic control degree (A1c <8 and ≥8%) and disease evolution (≤3 and >3 years), no differences were found in the studied molecules. hsCPR correlated with glucose (r=0.54, P=0.001), A1c (r=0.41, P=0.01), sE-S (r=0.47, P=0.004), and VCAM1 (r=0.41, P=0.02).

Conclusion: The increased levels of hsPCR and adhesion molecules suggest that a proinflammatory state associated with endothelial activation are present in children with T1D, enhancing the risk of cardiovascular disease.

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