ESPE Abstracts (2016) 86 RFC9.8

Micro RNA and Diabetic Nephropathy

Shereen Abdelghaffara, Fatma Elmougib, Sahar Abdelatyb, Yasmin Elshiwyb, Reham Elsayedb, Heba Abdelrahmanb, Hend Mehaweda, Heba Elgebalya, Sakinatalfouad Ahmeda & Peter Elalfya

aDepartment of Pediatrics, Cairo University, Cairo, Egypt; bDepartment of Clinical Pathology, Cairo University, Cairo, Egypt

Background: MicroRNAs (miRNAs) are short non-coding RNAs that repress target gene expression via post-transcriptional mechanisms.

Objective and hypotheses: To study the expression of miRNA-25, miRNA-216, miRNA-21, miRNA-93, miRNA-377 in a sample of 100 type 1 diabetic patients with and without microalbuminuria to probe their role in development of diabetic nephropathy.

Methods: Hundred type 1 diabetic patients, 50 with microalbuminuria and 50 without were recruited from the Diabetic Unit, Cairo University. Blood pressure measurement as well as HbA1C and lipid profile were assessed. Detection of mature miRNAs in serum was done by quantitative real-time reverse-transcription PCR (qRT-PCR).

Results: MicroRNA-21, miRNA93, miRNA25, miRNA were upregulated in 36.6, 76.7 and 30% respectively and downregulated in 60, 20 and 50% respectively of patients with microalbuminuria. miRNA-216 was upregulated in 20% and downregulated in 60% of patients with microalbuminuria.

Conclusion: Some microRNA- might have a protective role against development of microalbuminuria, while others may increase its risk. More studies are still needed to unmask the potential role of different micro-RNAs in development of diabetic nephropathy.

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