ESPE Abstracts (2018) 89 P-P1-082

The Comparison of the Occurrence of Beta Cells Autoantibody and Regulatory T Cells (CD4+CD25+FoxP3+) in Patients with Type 1 Diabetes Mellitus, their Siblings and Healthy Children

Joanna Sieniawska, Aleksandra Krzewska & Iwona Ben-Skowronek

Department of Pediatric Endocrinology and Diabetology Medical University of Lublin, Lublin, Poland

Background: Regulatory T cells (Treg) of phenotype CD4+CD25+FoxP3+ involves active suppression of excessive immune response. The population of Treg cells from patients with type 1 diabetes (DM1) have numeric and functional abnormalities. Although there are many reports of investigations on human and animal populations, the role of regulatory T cells in the development of type 1 diabetes is still unclear.

Objective and hypotheses: The aim of the study is to compare the population of regulatory T cells and the correlation between Treg cells and beta cells autoantibody in healthy siblings of children with DM1 to healthy children from non-diabetic families and to children with DM1.

Method: Peripheral blood mononuclear blood cells were obtained from 76 children with DM1, their siblings – 101, and 30 healthy children. Treg cells were characterized by flow cytometry FACSCalibur (Becton Dickinson, USA). The auto-antibodies were determined by ELISA. The results were analyzed with STATISTICA 10 PL.

Results: The number of regulatory T cells from diabetic patients was higher (average percentage 0.23±0.20) than that in the siblings (0.15±0.14) (P=0.004). There was no significant difference in the number of Treg cells between children with DM1 and the control group (0.19±0.15; P=0.11) and between siblings and the control group (P=0.09). The levels of anti IA2 and anti ZnT8 antibodies were statistically significant higher in siblings in comparison to the control group (anti IA2 Ab P=0.0000001; anti ZnT8 Ab P=0.00001). The level of anti-GAD in siblings was similar to that in the control group. There was no correlation between the number of Treg cells and the co-occurrence of beta cells auto-antibody.

Conclusion: The results suggest that regulatory T cells probably provide protection from development of disease and the dysfunction of Treg cells contributes to the autoimmune pathogenesis of type 1 diabetes.

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