ESPE Abstracts (2018) 89 P-P1-149

Assesment of SDF-1 and Ang-1 and Ang-2 in Children with Growth Hormone Deficiency before and after 1- year Therapy with Recombinant Growth Hormone

Beata Sawickaa, Marcin Moniuszkob, Kamil Grubczakb, Paulina Singhb, Urszula Radzikowskab, Paula Mikłaszb, Milena Dębrowskac, Hanna Borysewicz-Sańczyka & Artur Bossowskia


aDepartment of Pediatrics Endocrinology, Diabetes with the Cardiology Division; Medical University in Białystok, Białystok, Poland; bDepartment of Regenerative Medicine and Immune Regulation, Medical University in Białystok, Białystok, Poland; cDepartment of Hematology Diagnostic, Medical University in Białystok, Białystok, Poland


Introduction: Angiopoietins are necessary for development, differentiation and stabilization vessels progress. Angiopoient 1 (Ang-1) is responsible for vascular integrity, through stimulation of endothelial cell migration and adhesion, and inhibition of apoptosis. Action of angiopoietin 2 (Ang-2), in the absence of VEGF it Leeds to vascular regression, but in the presence of high VEGF concentration it stimulates angiogenesis. Stromal derived factor (SDF-1) play an important role in stem cells mobilization from bone marrow to the peripheral blond, what increases as a result of tissue injury. During therapy recombinant growth hormone (rGH) in patients with growth hormone deficiency (GHD) supply of increasing growth factors and a lot of processes development of cells.

Aim: The aim of the study was to estimate the concentration of angiopoietins 1 (Ang-1) and 2 (Ang-2) and stromal derived factor (SDF-1) in children with growth hormone deficiency before and after 1-year therapy with recombinant growth hormone.

Materials and methods: Anthropometric parameters (height, weight, BMI) and levels of angiopoient (Ang-1 and Ang-2) and stromal derived factor (SDF-1) were measured in 32 children with GHD before and during GH therapy. The control group comprised 16 healthy, age and sex matched children. Ang-1, Ang-2 and SDF-1 levels were determined with ELISA.

Results: Comparing to control group SDF-1 level decreases statistically significant (P<0.05) in the group with GHD and was demonstrated tendency to slightly decrease without statistical significance (P<0.05) in group treated with GH. Without statistically significant correlations (P<0.05) Ang-1 and Ang-2 decrease in group with GHD comparing to control group. Increasing levels of Ang-1 and Ang-2 (Ang2>Ang-1) was observed after 1-year therapy.

Conclusion: In conclusion, GHD connect with decreasing stromal derived factor (SDF-1) and angiopoient and play an important role in impaired regeneration and development new cells. SDF-1, Ang-1 and Ang-2 could be monitoring of patients response to therapy with GH.