ESPE Abstracts (2019) 92 P1-117

The Role of Rat Hypothalamus Kisspeptin, Neurokinin and their respective Receptors in the Prolactin-Infertility Interaction

Eylul Akbal Isik1, Inayet Nur USLU1, Gulsevinc AY1, Nesrin CETINEL1, Gamze COMERTPAY1, Hale OKSUZ1, Deniz BARC1, Kubra AKILLIOGLU2, Ali Kemal TOPALOGLU3, Mehmet Bertan YILMAZ1


1Cukurova University, Department of Medical Biology, Adana, Turkey. 2Cukuova University, Department of Medical Physiology, Adana, Turkey. 3Cukurova University, Department of Pediatrics, Adana, Turkey


Aim: In this study, we investigated whether there was a relationship between prolactin hormone (PRL), whose excess levels lead to infertility, and expression of Kisspeptin (Kiss1), Neurokinin (Tac2) and their respective Receptors.

Material and Methods: In our study, 12-16 weeks old 40 female Wistar Albino rats were classified into three groups; control group (n=10), physiological saline (SF) (n=10), and metaclopramide group (n=20), with high prolactin levels. Blood prolactin levels of all groups were measured using the ELISA immunoassay kit after two weeks of administration. Kiss1, Kiss1 Receptor (Kiss1R), Tac2, Tac2 Receptor (Tac2R) genes RNA expression were measured from hypothalamus.

Results: We found that prolactin level was highest in Metaclopramide group and lowest in the control group. When prolactin levels were compared in groups, a statistically significant difference was found between the groups (P <0.001). In Group 3, where PRL levels increased, the expression values of Kiss1, Kiss1R, and Tac2 genes were significantly decreased compared to the other two groups (P <0.001). There was no statistically significant difference between the groups in terms of expression values of Tac2R gene (P = 0.052). However, it can be inferred that this difference between the groups was borderline significant depending on the value of p.

Conclusion: In light of our findings, we think that prolactin increase may have adverse effects on reproduction by means of neuropeptides and our study will contribute in understanding the molecular basis of infertility problems caused by increased prolactin levels.