ESPE Abstracts (2019) 92 P1-197

You are What You Eat: Preliminary Evidence of Associations Between Dietary Habits and Oral Microbiota Composition in Early Childhood

Mélanie Henderson1,2, Belinda Nicolau3, Andraea Van Hulst3, Gabrielle Simoneau1,3, Tracie A. Barnett1,4, Vicky Drapeau5, Angelo Tremblay5, Marie-Eve Mathieu2, Gilles Paradis3, Michael Zappitelli6, Thibaut Varin7,5, André Marette7,5


1CHU Sainte-Justine, Montréal, Canada. 2Université de Montréal, Montréal, Canada. 3McGill University, Montréal, Canada. 4Centre INRS - Institut Armand-Frappier, Laval, Canada. 5Université Laval, Québec, Canada. 6University of Toronto, Toronto, Canada. 7IUCPQ, Québec, Canada


Background: Oral microbiota composition and diversity differ between obese and non-obese individuals. However, the associations between lifestyle habits (implicated in the pathogenesis of obesity) and the oral microbiota remain uncertain, particularly among children.

Objective: To explore the associations between oral microbiota diversity and lifestyle habits among 8-10 year-old children.

Methods: Data stem from the baseline assessment of the QUALITY cohort, a prospective cohort study of 630 children aged 8-10 years at recruitment, with a parental history of obesity. Lifestyle habits assessed include: physical activity by 7-day accelerometry, self-reported screen time, and dietary intake by 3 non-consecutive 24h dietary recalls. Fitness was measured by VO2peak. 16S-rRNA based microbial profiling of oral plaque samples obtained from 80 participants (40 normal weight, 40 overweight/obese) were performed to determine the diversity of the oral microbiota. Measures of diversity include Observed OTUs, Chao1, Shannon and Simpson reciprocal indices. Pearson's correlations assessed associations between diversity indices and lifestyle habits.

Results: Percent carbohydrate intake was positively correlated with all measures of diversity (Obs OTUs r=0.22, P=0.06; Chao1 r=0.23, P=0.042; Shannon r=0.19, P=0.096; Simpson reciprocal r=0.20, P=0.076). Conversely, while not reaching statistical significance, modest negative correlations between total dietary fat and saturated dietary fat consumption and measures of oral microbiota diversity were noted (r = -0.14 to -0.17 across all indices). Physical activity, fitness and screen time were not associated with oral microbiota diversity at 8-10 yr.

Conclusions: These preliminary findings suggest that dietary intake in childhood is associated with the bacterial diversity of the oral cavity