ESPE Abstracts (2019) 92 P1-345

ANGPTL-4 in Children and Adolescents: Relation to Gender, Puberty and Obesity

Silvia Barja-Fernández 1,2,3, Cintia Folgueira1,4,3, Cecilia Castelao1,3, Verónica Pena-León1,3, Patricia González-Saenz1,3, Rocío Vázquez-Cobela5, Concepción M Aguilera6,7, Mercedes Gil-Campos8,9, Gloria Bueno10,11, Ángel Gil6,7, Luis Moreno12,11, Manuel Ruiz-Piñon1,13, María García-Palacios1,14, Felipe F Casanueva15,3, Carlos Dieguez4,3, Rubén Nogueiras4,3, Luisa M. Seoane1,3, Rosaura Leis5,3

1Endocrine Physiopathology group, Health Research Institute of Santiago de Compostela, Santiago de Compostela, Spain. 2Pediatrics department, University of Santiago de Compostela, Santiago de Compostela, Spain. 3CIBER Fisiopatología Obesidad y Nutrición, ISCIII, Santiago de Compostela, Spain. 4Department Physiology, CIMUS- USC, Santiago de Compostela, Spain. 5Pediatrics Department. GI Nutrición Pediátrica (IDIS, CHUS). Galician Human Development, Growth and Nutrition Research Unit, Universidad Santiago de Compostela (USC), Santiago de Compostela, Spain. 6Biochemistry and molecular biology II Department, University Granada, Granada, Spain. 7CIBER Fisiopatología Obesidad y Nutrición, ISCIII, Granada, Spain. 8Paediatric Research and Metabolism Unit, Reina Sofia University Hospital, Cordoba, Spain. 9CIBER Fisiopatología Obesidad y Nutrición, ISCIII, Cordoba, Spain. 10Pediatric Department, Lozano Blesa University Clinical Hospital, Zaragoza, Spain. 11CIBER Fisiopatología Obesidad y Nutrición, ISCIII, Zaragoza, Spain. 12Public Health Department, University Zaragoza, Zaragoza, Spain. 13Operative Dentistry and Endodontics, USC, Santiago de Compostela, Spain. 14Department Pediatric Surgery, USC, Santiago de Compostela, Spain. 15Cellular and molecular endocrinology lab, USC., Santiago de Compostela, Spain

Introduction: Preclinical models demonstrated that Angiopoietin-like protein 4 (ANGPTL-4) regulates lipid metabolism and affects energy homeostasis. However, no data exist regarding its involvement in childhood and adolescence, periods of life with important metabolic changes.

Objectives: We aimed to investigate circulating levels of ANGPTL-4 in children and adolescents and its relationship with gender, puberty and obesity.

Material and Methods: Plasma ANGPTL-4 levels were analysed in 150 Caucasian children (72) and adolescents (78). The sample was classified as obese (percentile ≥ 95th; n = 77) and normal-weight (n = 73) by using the standard definition from Cole et al. The concentration of ANGPTL-4 was measured using an ELISA kit (Human ANGPTL4 ELISA kit SK00309-01; Aviscera Bioscience) and statistical analyses were performed using the SPSS software.

Results: Girls and boys showed similar concentrations in plasma levels of ANGPTL-4. The puberty did not modify the ANGPTL-4 circulating levels. However, ANGPTL-4 was significantly reduced in children and adolescents with obesity compared to lean participants. This reduction was independent of the gender and sexual maturation. Moreover, ANGPTL-4 was negatively correlated with BMI, body weight and waist circumference.

Conclusion: This study reveals that ANGPTL-4 levels are deregulated in children and adolescents with obesity independently of the gender and puberty.

Acknowledgments: CIBERobn, ISCIII (PI18/00998, PI15/01272, PI11/02042, PI16/01301, PI16/00871), FEDER, Fundación Mutua Madrileña.