ESPE Abstracts (2019) 92 P1-43

Association of Biomarkers of Endothelial Dysfunction with MicroRNAs Levels in Overweight and Obese Adolescentes

Fengyang Huang1, Malinalli Brianza-Padilla2,3, Herlinda Bonilla-Jaime4, Blanca Estela del-Río-Navarro5, Santiago Villafaña-Rauda6, Rodrigo Romero-Nava1, Fausto Sánchez-Muñoz3


1Laboratorio de Investigación en Farmacología, Hospital Infantil de México Federico Gómez, Mexico City, Mexico. 2Posgrado en Biologia Experimental, División de Ciencias Biológicas y de la Salud, Universidad Autonoma, Metropolitana Iztapalapa, Mexico City, Mexico. 3Departamento de Inmunología, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, Mexico. 4Biologia de la Reproducción, División de Ciencias Biológicas y de la Salud, Universidad Autonoma, Metropolitana Iztapalapa, Mexico City, Mexico. 5Departamento de Alergía, Hospital Infantil de Mexico Federico Gomez, Mexico City, Mexico. 6Division de Posgrado Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico City, Mexico


Background and Aims: MicroRNAs play important regulatory roles in cholesterol homeostasis and endothelial dysfunction. The aim of present study was to characterize the expression of miRNAs in overweight/obese adolescents and to identify the possible correlation with metabolic profile and inflammatory biomarkers.

Methods: 79 overweight and obese adolescents aged 13.0±2.0 years and 28 normal weight adolescents aged 13.7±2.0 years were recruited. The concentrations of biomarkers of endothelial dysfunction (sE, sICAM-1, PAI-1, and fibrinogen) were measured by ELISA and the serum relative expression of miRNAs (miR-223, miR-33a, and miR-126) were determined by real-time quantitative PCR.

Results: The overweight and obese children presented increased levels of sE, sICAM-1, PAI-1, and fibrinogen compared with normal weight subjects. The overweight and obese adolescents also showed higher levels of miR-223 and miR-33a than normal weight subjects. No different level of miR-126 was observed between studied groups. By Spearman analysis, the levels of miR-223 positively correlated with age, weight, BMI, insulin, HOMA and PAI-1. The levels of miR-33a correlated with sICAM-1 in a positive manner and with HDL in a negative manner. The levels of miR- 126 positively correlated with age and triglyceride.

Conclusion: The present study showed alterations of two miRNAs related to cholesterol homeostasis and endothelial activation in overweight and obese adolescents as compared with normal weight subjects. The correlation among miRNAs, metabolic profile, and inflammatory biomarkers supported the use of some miRNAs extracted from serum samples as potential predictive tool forobesity.

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