ESPE Abstracts (2019) 92 P3-231

Falsely Elevated Serum Sex Steroid Hormones in a Girl with Premature Adrenarche

Lavinia La Grasta Sabolić1, Marija Požgaj Šepec1, Ivana Zec2, Dario Mandić3,4, Gordana Stipančić1


1Department of Pediatrics, Sestre milosrdnice University Hospital Center, Zagreb, Croatia. 2Laboratory of Endocrinology, Clinics of Oncology and Nuclear Medicine, Sestre milosrdnice University Hospital Center, Zagreb, Croatia. 3Institute of Clinical Laboratory Diagnostics, Osijek University Hospital, Osijek, Croatia. 4Department for Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia


Background: Laboratory evaluation is crucial for accurate assessment of patients with endocrine disorders. When clinical picture is in obvious contradiction with laboratory results, one should suspect and prove analytical interference.

Case presentation: a 6.7-year-old girl presented with sexual and axillary hair accompanied by adult-type body odor. She was tall (height SDS 2.6), with no other signs of virilisation and no breast development. Her bone age was slightly advanced (7.5 years), and laboratory workup showed markedly elevated levels of dehydroepiandrosterone sulphate (DHEAS), elevated testosteron and appropriate level of 17-hydroxyprogesterone (17-OHP) measured by electrochemical luminescence immunoassay (ECLIA). Subsequent investigations confirmed previous laboratory results, but also revealed elevated serum estradiol with no evidence of estrogen effect on uterus and no adrenal or adnexal mass. Cortisol level was unremarkable. During one year folow up, there were no changes in girl's clinical appearance, while levels of her sex steroids determined by ECLIA fluctuated from undetectable or slightly elevated to markedly elevated. There were no apparent risk factors for analytic interference (no biotin supplementation or drugs, negative rheumatoid factor, no hypergammaglobulinemia). When samples were reanalyzed with the addition of a blocking agent, significantly lower levels of serum sex steroids were obtained, while liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed sex steroids appropriate for age.

Conclusion: Laboratory interference is a drawback in hormonal testing. Clinicians should have that in mind when faced with laboratory results discordant with patient's clinical presentation. Elevation of multiple sex steroid hormones in a prepubertal girl due to laboratory interference in immunoassay was finally unmasked by LC-MS/MS.

Article tools

My recent searches

No recent searches.