ESPE Abstracts (2019) 92 T1

Circulating miR-451a: A Biomarker to Guide Diagnosis and Treatment of Polycystic Ovary Syndrome in Adolescent Girls

Marta Díaz1,2, Judit Bassols3, Abel López-Bermejo4, Francis de Zegher5, Lourdes Ibáñez1,2


1Institut de Recerca Pediàtric Hospital Sant Joan de Déu, University of Barcelona, Barcelona, Spain. 2Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), ISCIII, Madrid, Spain. 3Maternal-Fetal Metabolic Research Group, Girona Institute for Biomedical Research (IDIBGI), Girona, Spain. 4Pediatric Endocrinology Research Group, Girona Institute for Biomedical Research (IDIBGI) and Dr. Josep Trueta Hospital, Girona, Spain. 5Department of Development & Regeneration, University of Leuven, Leuven, Belgium


Polycystic ovary syndrome (PCOS) is a prevalent disorder in adolescent girls, commonly driven by hepato-visceral fat excess, usually presenting with hirsutism and menstrual irregularity, and often followed by subfertility and type 2 diabetes.

We studied the miRNA profile of adolescent girls with PCOS, and the effects of randomized treatment with an oral contraceptive (OC) or with spironolactone-pioglitazone-metformin (SPIOMET, aiming at loss of hepato-visceral fat excess) for 1 year. Post-treatment ovulation rates were assessed by salivary progesterone. The miRNA profiling was performed by RNA sequencing, differentially expressed miRNAs being validated by qRT-PCR in 13 control and 31 PCOS girls.

Girls with PCOS had markedly reduced concentrations of circulating miR-451a, miR-652-3p, miR-106b-5p and miR-206; pathway enrichment analysis showed that these miRNAs target genes involved in energy homeostasis and cell-cycle control. In the present study, miR-451a could diagnose PCOS (versus a healthy condition) with 100% sensitivity and 100% specificity. SPIOMET (but not OC) was accompanied by on-treatment normalization of the miRNA profile in PCOS girls; miR-451a concentrations after 1 year on OC or SPIOMET treatment associated closely (r=0.66; P<0.0001) with post-treatment ovulation rates.

In conclusion, circulating miR-451a may become a biomarker contributing to guide the diagnosis and treatment of PCOS in adolescent girls.

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