ESPE Abstracts (2016) 86 P-P1-640

Analysis of Correlation between Stem Cells (CD133+/CD45+ and CD133+/CD45−) and Anthropometric Parameters of Children with Growth Hormone/Primary Insulin-Like Growth Factor 1 Deficiency

Beata Sawickaa, Marcin Moniuszkob, Kamil Grubczakb, Paulina Singhb, Urszula Radzikowskab, Paula Mikłaszb, Milena Dębrowskac & Artur Bossowskia

aDiabetes with the Cardiology Division, Department of Pediatrics Endocrinology, Medical University in Bialystok, Bialystok, Poland; bDepartment of Regenerative Medicine and Immune Regulation, Medical University in Bialystok, Bialystok, Poland; cDepartment of Hematology Diagnostic, Medical University in Bialystok, Bialystok, Poland

Introduction: Hematopoetic progenitor stem cells (HSCs, CD133+/CD45+) and very small embryonic-like stem cells (VSELs, CD133+/CD45) can differentiate into specific immune cells. Some studies suggest that levels of HSCs and VSELs change during therapy with growth hormone (GH) or insulin-like growth factor 1 (IGF-1). GH deficiency (GHD), an endocrine disease connected with insufficient production of GH by pituitary gland, is treated with synthetic GH. IGF-1 is main growth factor, secreted under the influence of GH. Primary IGF-1 deficiency syndrome (PIGFDS) may be caused by genetic defects and it is treated with IGF-1.

Aim: The aim of the study was to estimate the concentration of very small embryonic-like cells (VSELs) delineated by Lin-CD133+CD45 phenotype and hematopoietic stem/progenitor cells characterized by Lin-CD133+CD45+ phenotype in relation to treatment with GH or IGF-1. To assess correlation between the level of HSCs and VSELs and anthropometric parameters.

Materials and methods: Anthropometric parameters (height, weight, BMI) and HSCs and VSELs levels were measured in 32 children with GHD during GH therapy and 4 with PIGFDS during IGF-1 therapy. Mean age 12 years old. The control group comprised 16 healthy, age and sex matched children. HSCs and VSELs levels were determined with flow cytometry.

Results: Comparing to control group HSCs level increases statistically significant (P<0,05) in the group treated with GH but tendency to increase without statistical significance was demonstrated by VSELs in both study groups and by HSCs in IGF-1 treated group. Statistically significant correlations (P<0,05) between stem cells levels and anthropometric parameters were observed in both GH (HSCs and weight, VSELs and height) and IGF-1 (VSELs and BMI, VSELs and height) treated patients.

Conclusion: In conclusion, GH and IGF-1 mobilize stem cells. VSELs and HSCs could be monitoring markers of patients response to therapy.