ESPE Abstracts (2016) 86 FC5.2

Management of Disorders of Insulin Secretion

Diabetes and Insulin Injection Modalities: Effects on Hepatic Expression and Activity of 11β-Hydroxysteroid Dehydrogenase Type 1 in Juvenile Diabetic Rats

Veronica Rougeona,b, Marie-Pierre Moisanc, Nicole Barthee, Marie-Christine Beauvieuxd, Nathalie Marissal-Arvyc & Pascal Barata,b


aUniversité Bordeaux, Nutrition et Neurobiologie Intégrée, Bordeaux, France; bCHU Bordeaux, Hôpital des Enfants, Bordeaux, France; cINRA, Nutrition et Neurobiologie intégrée, Bordeaux, France; dLaboratoire de Biochimie de l’Hôpital Haut-Lévêque, Pessac, France; eINSERM U1026 BioTis, Bordeaux, France

Background and hypotheses: Recent results showing elevated tetrahydrocortisol/tetrahydrocorticosterone ratio (THFs/THE) in morning urines of diabetic children compared to controls suggest an increased nocturnal activity of 11 β-hydroxysteroid dehydrogenase type 1 (11β-HSD1). We made the hypothesis that a reduced inhibition of hepatic 11β-HSD1 activity by exogenous insulin, due to its subcutaneous (SC) administration and absence of first hepatic passage, could explain these observations.

Objective: Our main objective was to compare 11β-HSD1 activity and expression in the liver of young diabetic rats, treated either by subcutaneous (SC) or intraperitoneal (IP) insulin. Our hypothesis was that hepatic 11β-HSD1 is less inhibited by SC insulin than by IP insulin.

Method: We formed 4 groups of juvenile Wistar rats: 12 controls, 36 streptozotocin-induced diabetic rats among them 12 were not treated (NT), 12 were treated with SC insulin and 12 with IP insulin. We measured 11β-HSD1 expression and activity in the liver at 7 weeks of life.

Results: Hepatic gene expression and activity of 11β-HSD1 were increased in the untreated diabetic group compared to controls. Although diabetes was controlled equally whatever the route of insulin administration, 11β-HSD1 gene expression and activity were only decreased in the IP group.

Conclusion: Ours results support that hepatic first pass is needed for 11β-HSD1 hepatic inhibition.

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