ESPE2018 Poster Presentations Growth & Syndromes P1 (30 abstracts)
Analysis of Osteoblats Precursors in Girls with Turner Syndrome
Giacomina Brunetti a , Mariangela Chiarito b , Laura Piacente b , Gabriella Aceto b , Silvia Colucci a , Graziana Colaianni c , Maria Grano c , Gabriele D’Amato d & Maria Felicia Faienza b
aDepartment of Basic Medical Sciences, Neuroscience and Sense Organs, Section of Human Anatomy and Histology, University ‘A. Moro’ of Bari, Bari, Italy; bDepartment of Biomedical Sciences and Human Oncology, Pediatric Section, University ‘A. Moro’ of Bari, Bari, Italy; cDepartment of Emergency and Organ Transplantation, University ‘A. Moro’ of Bari, Bari, Italy; dNeonatal Intensive Care Unit, Di Venere Hospital, Bari, Italy
Background and aim: Subjects with Turner Syndrome (TS) show low cortical bone mineral density (BMD), osteoporosis and risk of fractures. Previously, we demonstrated the enhanced spontaneous osteoclastogenesis in girls and young women with TS before and after pubertal induction with hormonal replacement therapy (HRT). The bone resorption observed in girls before puberty induction seems to be supported by the high FSH serum levels observed at prepubertal stage, while in young women on continuous HRT the effects on osteoclasts seem to be mediated mostly by high RANKL levels. We aimed to investigate if osteoblast differentiation/activity could also be impaired in girls with TS.
Method: Markers of bone remodelling were measured in sera of 10 girls with TS (median age 5.2±1.2) and 10 age matched controls. Bone mineral density was evaluated by DEXA. The percentage of circulating osteoblast precursors CD34+/CD45-/Osteocalcin (OCN)+ cells was evaluated by flow cytometry.
Results: Lumbar spine BMD-Z-score was reduced in 50% TS patients, together with levels of 25-OH Vitamin D. In sera of TS patients we also detected a significant slight increase of the levels of the osteoblastogenesis inhibitor sclerostin compared with the controls (28.45±9.43 vs 20.99±9.31, P<0.01 respectively). Consistently, we also detected a significant low percentage of circulating osteoblast precursors CD34+/CD45-/Osteocalcin (OCN)+ cells in TS patients with respect to the controls (1.1±. 0.2 vs 3.5±0.5 P<0.01, respectively). The percentage of circulating osteoblast precursors positively correlated with the reduced lumbar spine BMD-Z-score (r=0.42, P<0.01) and with the low levels of 25-OH Vitamin D (r=0.45, P<0.01).
Conclusions: In TS patients the low percentage of circulating osteoblast precursors CD34+/CD45-/Osteocalcin (OCN)+ cells together with the high levels of sclerostin sustain the involvement of osteoblasts in the impaired bone remodeling associated to TS.
Article tools
My recent searches
My recently viewed abstracts
Authors
ESPE Abstracts
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more