ESPE Abstracts (2018) 89 RFC7.4

Expression of MIR-576-5p in Umbilical Cord as a Novel Biomarker for the Identification of Catch-up Growth in Small-For-Gestational-Age Infants

Judit Bassolsa, Berta Mas-Paresa, Alexandra Bonmatib, Silvia Xargay-Torrenta, Gemma Carreras-Badosac, Esther Lizarraga-Mollinedoa, Jose-Maria Martinez-Calcerradad, Francis de Zeghere, Lourdes Ibañezf & Abel Lopez-Bermejoa,b

aInstitut d’Investigació Biomèdica de Girona (IDIBGI), Girona, Spain; bHospital Dr. Josep Trueta, Girona, Spain; cUniversity of Tartu, Tartu, Estonia; dInstitut de Medicina Legal (IMLC), Girona, Spain; eUniversity of Leuven, Leuven, Belgium; fInstitut de Recerca Pediàtrica Sant Joan de Déu, Barcelona, Spain

Background: Early catch-up growth, between birth and age two years, in infants born small-for-gestational-age (SGA) is a risk factor for the development of cardiometabolic diseases in adulthood. The basis and mechanisms underpinning catch-up growth in SGA newborns are unknown.

Objectives: We aimed to investigate the catch-up predictive ability of cord blood miRNAs in SGA infants.

Methods: MicroRNA PCR Human Panels were used to study the miRNA profile in umbilical cord tissue of 5 SGA infants with catch-up (SGA-CU), 5 SGA infants without catch-up (SGA-nonCU) and 5 control infants (appropriate-for-gestational-age, AGA). The miRNA with differential expression between the study groups were validated in a cohort of 64 infants (24 SGA-CU, 18 SGA-nonCU and 22 AGA) and correlated with anthropometric and metabolic parameters (weight and height gain, body composition and fat distribution) at 1 and 6 years of age.

Results: The miR-501-3p, miR-576-5p, miR-770-5p and miR-876-3p associated with increased weight, height, weight catch-up and height catch-up at 1 year of age (all P<0.05); and the miR-374b-3p, miR-548c-5p, miR-576-5p y miR-99a-5p associated with increased weight, height, waist circumference and renal fat (all P<0.05) at 6 years of age. Multivariate analysis showed that miR-576-5p was predictor of weight catch-up (β=−0.474, P=0.001; R2=19.1) and height catch-up (β=0.459, P=0.01; R2=0.652) at 1 year of age; and waist circumference (β=0.459, P=0.01; R2=0.652) and renal fat (β=0.455, P=0.03; R2=0.207) at 6 years of age. In Silico studies showed that miR-576-5p participates in the regulation of inflammatory, growth and proliferation signaling pathways.

Conclusion: miR-576-5p could be a novel biomarker for the early identification of catch-up growth in SGA infants. miR-576-5p may also contribute to the regulation of postnatal growth and influence the risk for cardiometabolic diseases associated with postnatal growth.