ESPE Abstracts

Key words: Type 1 diabetes mellitus; Intestinal flora; metagenomic sequencing; cytokines; Autoimmunity

Objective: This study aims to verify the effect of intestinal microbiota diversity on the disease development of T1DM mice model, and to explore the mechanism of intestinal microbiota in the development of type 1 diabetes mediated by related cytokines.

Method: T1DM mouse model was established and different gut bacterial humoral transplants were performed. Differential strains were validated and screened using high-throughput cytokine chip technology, and differential proteins and enriched immune related signaling pathways were screened. The levels of key cytokines in the signaling pathway were detected using ELISA technology, and correlation and regression analysis were performed with the differential strains.

Result: The differences between T1DM and non T1DM intestinal cultures were validated using a mouse model, and different cultures of Klebsiella_grimontii, Absiella_dolichum and Bifidobacterium_longum were screened. High throughput cytokine detection showed elevated levels of pro-inflammatory factors in the IL-17 signaling pathway, with Absiella_dolichum, Bifidobacterium_longum and cytokine IL-17A levels are negatively correlated, while Klebsiella_grimontii strains showed a positive correlation.

Conclusion: The change of Gut microbiota may further promote the occurrence and development of T1DM by over activating IL-17 pathway.