hrp0082fc1.3 | Adrenal | ESPE2014

Genetic Engineering Using TALENs to Study the Redox Regulation of Steroidogenesis in vivo

Griffin Aliesha , Parajes Silvia , Taylor Angela , Mueller Ferenc , Krone Nils

Background: Transcription activator-like effects nucleases (TALENs) have recently been developed as an efficient method for in vivo genome engineering. Zebrafish are becoming an increasingly popular model to study translational aspects in endocrinology. The redox cofactor ferredoxin (FDX1) is essential for mitochondrial cytochrome P450 (CYP) enzymes including those required for steroidogenesis. In vitro, FDX1 modifications influence the catalytic rate of ster...