hrp0084p1-15 | Bone | ESPE2015

Lithium Chloride Prevents Glucocorticoid-Induced Growth Failure in Cultured Foetal Rat Metatarsal Bones

Soucek Ondrej , Zaman Farasat , Savendahl Lars

Background: Glucocorticoids (GCs) are frequently used to treat numerous chronic diseases in children. Beside their desired anti-inflammatory and immunosuppressive effects, GCs are well known to cause osteoporosis and impaired linear bone growth. These serious side effects of GCs have at least partially been linked to impairment of Wnt/β-catenin signalling. There is no therapy available to rescue from the undesired skeletal effects of GCs.Objective a...

hrp0084p3-1014 | Growth | ESPE2015

Altered Gene-Expression in Human Growth Plate Cartilage Tissue Exposed to Dexamethasone

Cedervall Therese , Zaman Farasat , Savendahl Lars

Background: Synthetic glucocorticoids (GCs) are widely used drugs but their suppression of growth in pediatric patients is a well-known problem. Different mechanisms have been suggested but we still do not know the sensitivity of human growth plate cartilage to GC treatment. Here in this study, we have investigated the direct effects of GC treatment on the expression of crucial genes in the growth plate, such as collagen-2A1, osterix (Osx), and transforming growth factor &#946...

hrp0089fc2.5 | Bone, Growth Plate & Mineral Metabolism 1 | ESPE2018

Radial ESWT Stimulates Longitudinal Bone Growth in Cultured Rat Fetal Metatarsal Bones

Ramesh Sowmya , Zaman Farasat , Madhuri Vrisha , Savendahl Lars

Background: Extracorporeal shock wave therapy (ESWT) is widely used in the clinic for non-union of fractures. However, effect of ESWT on longitudinal bone growth has not been well studied. We explored the in vitro and in vivo effects of low/high dose radial shock wave treatment (SWT) on growth plate (GP) cartilage. A positive or negative effect on growth could be harnessed for therapeutic growth modulation while no change establishes safety.<p class="abst...

hrp0084p1-17 | Bone | ESPE2015

Humanin Prevents Undesired Apoptosis of Chondrocytes without Interfering with the Anti-Inflammatory Effect of Dexamethasone in a Model of Arthritis

Sederquist Bettina , Aulin Cecilia , Zaman Farasat , Savendahl Lars

Background: Glucocorticoids (GCs) are widely used for treatment of inflammatory and autoimmune conditions. Prolonged use of GCs, however, has several negative side effects, including bone growth impairment in children. Previous studies have shown that GC-induced apoptosis in growth plate chondrocytes is mediated by inhibition of the PI3K–Akt signaling pathway and activation of the pro-apoptotic protein Bax. Humanin, a small mitochondrial derived peptide, has shown promisi...

hrp0095p1-27 | Bone, Growth Plate and Mineral Metabolism | ESPE2022

Effects of Pharmacological Inhibition of BCL-2 on Linear Bone Growth

Velentza Lilly , Wickström Malin , Kogner Per , Zaman Farasat , Sävendahl Lars

Introduction: The survival rates for childhood cancer have significantly increased over the past decades but the management of treatment-related complications remains challenging. Thus, the introduction of novel drugs requires careful investigation of potential off-target side effects. The developing skeleton is severely affected by the anticancer treatment which may result in growth retardation and short stature. Venetoclax is a selective Bcl-2 inhibitor whic...