hrp0084np2.2 | New Perspective Session 2 | ESPE2015

Astrocytes and Neuroendocrine Control

Chowen Julie A

Glial cells are the most abundant cell type in the CNS. Although they were originally thought to only play a supportive role for neurons, it is now clear that glial cells are involved in all aspects of brain function. Understanding how glia, and in particular astrocytes, tanycytes and microglia, participate in the neuroendocrine control of metabolic homeostasis has come to the forefront in recent years. This is largely due to the observation that high fat diet-induced hypothal...

hrp0092fc15.6 | Late Breaking Abstracts | ESPE2019

Leptin Influences the Down-Regulation of UCP-1 Expression in Brown Adipose Tissue During Negative Energy Balance

Barrios Vicente , Canelles Sandra , Frago Laura M. , Chowen Julie A. , Argente Jesús

Background: The GH/IGF-I axis is involved in metabolic control and studies suggest that IGF-I deficiency and subsequent changes in IGF-I signaling in brown adipose tissue (BAT) modifies its thermogenic capacity. Food restriction reduces thermogenic capacity in BAT, while leptin stimulates thermogenesis through uncoupling protein 1 (UCP-1) induction. Leptin and IGF-I maintain important crosstalk in different tissues, but whether these two hormones interact to r...

hrp0089fc4.3 | GH & IGFs | ESPE2018

The Reduction in Longitudinal Growth Induced By PAPP-A2 Deficiency is Associated with Reduced Body Weight, Increased Energy Expenditure and Behavior Modification

Suarez Juan , Rivera Patricia , Vargas Antonio , Rubio Leticia , de Fonseca Fernando Rodriguez , Chowen Julie , Argente Jesus

Background: Pregnancy associated plasma protein (PAPP)-A2 is an insulin-like growth factor (IGF) binding protein (BP) protease that regulates IGF-1 availability, affecting postnatal growth. We have recently reported the first mutations in human PAPP-A2 causing short stature and changes in bone size and mineral density. However, the IGF system is involved in diverse physiological functions and to date it is unknown how mutations in PAPP-A2, which significantly reduce f...

hrp0082p2-d1-371 | Fat Metabolism & Obesity | ESPE2014

Replacement of the Neonatal Leptin Surge During Maternal Deprivation Normalizes Some Endocrine Parameters but Exacerbates Others

Mela Virginia , Lopez-Rodriguez Alvaro , Penasco Sara , Barrios Vicente , Argente Jesus , Viveros Maria-Paz , Chowen Julie A

Introduction: Maternal deprivation (MD) during neonatal life has diverse long-term effects, including modification of metabolism. Some of these effects are sexually dimorphic. We have previously reported that MD in rats blocks the physiological neonatal leptin surge, which could underlie the long-term metabolic changes.Hypothesis: We hypothesized that replacement of leptin during MD would normalize long-term endocrine changes.Metho...

hrp0084p3-859 | Fat | ESPE2015

Long-Term Effects of Neonatal Over-Nutrition on Metabolic Equilibrium are Age and Sex Dependant

Argente-Arizon Pilar , Ros Purificacion , Diaz Francisca , Fuente-Martin Esther , Barrios Vicente , Chowen Julie A , Argente Jesus

Background: Neonatal over-nutrition (NON) can have a long-term effects on energy homeostasis and some of these effects may be sexually dimorphic.Objective and hypotheses: We aimed to determine how NON affects body weight (BW), body composition and cytokine levels throughout development and if these changes are sexually dimorphic. We hypothesised that the effects would be both age and sex dependant.Methods: At birth, Wistar rats wer...

hrp0089p1-p113 | Fat, Metabolism and Obesity P1 | ESPE2018

Maternal Resveratrol Intake During Pregnancy and Lactation Modulates the Long-term Metabolic Effects of Maternal Nutrition on Offspring Depending on the Sex and Diet

Ros-Perez Purificacion , Diaz Francisca , Freire Alejandra , Argente-Arizon Pilar , Argente Jesus , A. Chowen Julie

Maternal nutrition can have significant long-term consequences on energy homeostasis in the offspring. However, whether resveratrol, with antioxidant and anti-obesity actions, can improve the impact of poor maternal nutrition on offspring metabolism remains largely unknown. We hypothesized that resveratrol intake by dams would protect the offspring against the harmful effects of a maternal high fat diet (HFD). We also determined if resveratrol’s effects are diet and sex d...

hrp0089p1-p140 | Fetal, Neonatal Endocrinology and Metabolism P1 | ESPE2018

Sexual Dimorphism of IGF1 and IGF2 Expression in the Neonatal Rat Brain

Guerra-Cantero Santiago , Torrecilla Marta , Diaz Francisca , Argente Jesus , Chowen Julie

Insulin-like growth factor (IGF) 2 plays a fundamental role in prenatal growth and development. The IGF2 gene is imprinted, with the paternally inherited copy being active and the maternal copy being silenced in most tissues. During development, the expression of IGF2 is sexually dimorphic in some tissues and this is thought to be involved in the development of some sexually dimorphic features. For example, IGF2 expression is reported to be higher in the male brain co...

hrp0086p1-p447 | Fat Metabolism and Obesity P1 | ESPE2016

Neonatal Overnutrition Causes Sex and Age Dependant Long-Term Effects on Body Weight, Body Composition and Serum Triglyceride and Free Fatty Acid Levels

Argente-Arizon Pilar , Diaz Francisca , Fuente-Martin Esther , Chowen Julie Ann , Argente Jesus

Background: Neonatal over-nutrition (NON) can increase the propensity to become overweight and develop associated metabolic disturbances in later life. Moreover, some of these long-term effects are sexually dimorphic.Objective and hypotheses: We aimed to determine how NON affects body weight (BW), body composition and triglyceride (TG) and non-esterified fatty acids (NEFA) levels. We hypothesized that the effects would be both age and sex dependant.<...

hrp0082fc12.1 | Obesity | ESPE2014

High-Fat Diet Rapidly Triggers Circadian De-Synchronization of Clock Genes, Neuropeptides and Inflammation Mediators in the Hypothalamus of C57BL Mice

Hernandez-Nuno Francisco , Ruiz-Gayo Mariano , Diaz Francisca , Argente Jesus , Chowen Julie A

Background: Circadian disorganization of feeding behavior evoked by high fat diet (HFD) intake is suggested to be involved in the resulting weight gain and development of associated metabolic alterations and hypothalamic inflammation.Hypothesis: We hypothesized that this circadian alteration might be a consequence of rapid de-synchronization of different gene clusters relevant for metabolic control.Methods: We analyzed the circadia...

hrp0082p1-d1-110 | Fat Metabolism &amp; Obesity | ESPE2014

Free Fatty Acids Activate Hypothalamic Astrocytes in a Sexually Dimorphic Manner

Argente-Arizon Pilar , Fuente-Martin Esther , Diaz Francisca , Freire-Regatillo Alejandra , Argente Jesus , Chowen Julie A

Introduction: Obesity is known to associate with chronic systemic inflammation. However, hypothalamic inflammation also occurs in response to high fat diet (HFD)-induced obesity and is proposed to participate in central insulin/leptin resistance and the perpetuation of weight gain and systemic affectation. The weight gain and central responses to HFD differ between males and females. As hypothalamic glial cells are implicated in the central inflammatory response we hypothesize...