hrp0082fc1.1 | Adrenal | ESPE2014

Molecular mechanisms of nongenomic glucocorticoid actions: the role of human glucocorticoid receptor S-palmitoylation

Nicolaides Nicolas C , Roberts Michael L , Kino Tomoshige , Katsantoni Eleni , Sertedaki Amalia , Chrousos George P , Charmandari Evangelia

Background: In humans, glucocorticoids (GCs) regulate a broad spectrum of physiologic functions, exerting both genomic and nongenomic actions through their ubiquitously expressed glucocorticoid receptor (hGR). The rapid nongenomic actions of GCs are likely to be mediated by membrane hGRs that transduce the glucocorticoid signal via activation of kinases. S-palmitoylation plays an important role in plasma membrane localization and occurs through a highly conserved nine amino ac...

hrp0082p2-d1-373 | Fat Metabolism & Obesity | ESPE2014

Expression Levels of the Growth-Arrest-Specific Transcript 5 in Overweight and Obese Children and Adolescents

Fragouli Terpsihori , Sertedaki Amalia , Farakla Ioanna , Nicolaides Nicolas C , Dracopoulou Maria , Papassotiriou Ioannis , Chrousos George P , Charmandari Evangelia

Background: The noncoding RNA growth-arrest-specific transcript 5 (Gas5) is abundant in cells whose growth has been arrested owing to lack of nutrients or growth factors. Gas5 is a riborepressor of the glucocorticoid receptor, influencing cell survival and metabolic activities during starvation by inhibiting the latter’s transcriptional activity.Aim: To determine the expression levels of Gas5 in blood samples of obese, overweight and lean children a...

hrp0084p2-168 | Adrenals | ESPE2015

The Natural Mutant Receptor hGRαT556I Causes Primary Generalised Glucocorticoid Resistance Through Decreased Affinity for the Ligand and Impaired Interaction with the GRIP1 Coactivator

Nicolaides Nicolas C , Skyrla Eirini , Vlachakis Dimitrios , Psarra Anna-Maria G , Sertedaki Amalia , Kossida Sophia , Charmandari Evangelia

Background: Primary generalised glucocorticoid resistance (PGGR) is a rare condition characterised by tissue insensitivity to glucocorticoids owing to inactivating mutations of the hGR gene. A new case of PGGR was reported in a patient with an adrenal incidentaloma harboring a novel heterozygous point mutation in the hGR gene, which resulted in threonine (T) to isoleucine (I) substitution at amino acid position 556 of the receptor.Objec...

hrp0094p1-105 | Adrenal B | ESPE2021

The genetic etiology of ACTH-dependent aldosterone hypersecretion in hypertensive patients without Primary Aldosteronism

Mourtzi Niki , Sertedaki Amalia , Markou Athina , Piaditis Georgios , Katsanis Nicholas , Traeger-Synodinos Joanne , Tsigos Constantinos , Charmandari Evangelia ,

Introduction: Compelling evidence suggests that Primary Aldosteronism (PA) is much more prevalent than previously thought, accounting for more than 20% of patients with resistant hypertension. Although, major advances have been made in the field of genetics underpinning sporadic and familial forms of PA, the etiology of mild forms of PA is poorly understood. In previous studies, we identified a distinct cohort of patients without PA who exhibited ACTH-dependen...

hrp0094p1-106 | Adrenal B | ESPE2021

Plasma Proteomics in Healthy Subjects with Differences in Tissue Glucocorticoid Sensitivity Identifies a Novel Proteomic Signature

Nicolaides Nicolas C. , Makridakis Manousos , Stroggilos Rafael , Koniari Eleni , Papageorgiou Ifigeneia , Sertedaki Amalia , Zoidakis Jerome , Charmandari Evangelia ,

Background: Tissue sensitivity to glucocorticoids is characterized by significant inter-individual variation in terms of therapeutic response and susceptibility to several stress-related disorders. Proteomics approaches, combined with appropriate bioinformatics analysis, offer a comprehensive description of molecular phenotypes with clear links to human disease pathophysiology.Objective and Hypotheses: To investigate the usefulness of pl...

hrp0086p1-p7 | Adrenal P1 | ESPE2016

Transient Generalized Glucocorticoid Hypersensitivity Syndrome

Kyritsi Eleni Magdalini , Nicolaides Nicolas C , Lamprokostopoulou Agaristi , Xaidara Athina , Georgiadou Elizabeth , Dimitropoulou Vassiliki , Stefa Alketa , Sertedaki Amalia , Chrousos George P , Charmandari Evangelia

Background: Transient Generalized Glucocorticoid Hypersensitivity (TGGH) is a rare disorder characterized by increased tissue sensitivity to glucocorticoids and compensatory hypoactivation of the hypothalamic–pituitary–adrenal (HPA) axis. The condition itself and the underlying molecular mechanisms have not been fully elucidated.Objective and hypotheses: To present the clinical manifestations, endocrinologic evaluation and molecular studies in ...

hrp0082p1-d2-3 | Adrenals & HP Axis | ESPE2014

Transient Generalized Glucocorticoid Hypersensitivity: Clinical Manifestations, Endocrinologic Evaluation, and Transcriptomic Profile: the Potential Role of nf-κb

Nicolaides Nicolas C , Lamprokostopoulou Agaristi , Polyzos Alexandros , Kino Tomoshige , Katsantoni Eleni , Triantafyllou Panagiota , Christophoridis Athanasios , Katzos George , Drakopoulou Maria , Sertedaki Amalia , Chrousos George P , Charmandari Evangelia

Background: Transient generalized glucocorticoid hypersensitivity is a rare disorder characterized by increased tissue sensitivity to glucocorticoids and compensatory hypoactivation of the hypothalamic–pituitary–adrenal (HPA) axis. The condition itself and the molecular mechanisms that underlie its pathophysiology have not been elucidated as yet. Adenovirus 36 has been reported to cause obesity in various animal species.Objective and Hypotheses...

hrp0095p1-248 | Diabetes and Insulin | ESPE2022

Carbohydrate counting efficacy in glycaemic control among children and adolescents with type 1 diabetes mellitus: a systematic review and meta-analysis

Amalia Gassani , Faisal Putro Utomo Muhammad , Aman Bhakti Pulungan

Background: Carbohydrate counting is an essential part of comprehensive diabetes management and is required for children and adolescents with type 1 diabetes mellitus (T1DM), according to available guidelines. However, there is no specific evidence-grading for carbohydrate counting. This review was done to determine the effectiveness of carbohydrate counting in improving glycaemic control among children and adolescents with T1DM as measured by glycated haemogl...

hrp0089p2-p104 | Diabetes & Insulin P2 | ESPE2018

Age and Exocrine Pancreatic Enzyme Requirements are Major Determinants for Carbohydrate Metabolism Impairment in Children Affected with Cystic Fibrosis

Escudero Janire , Uribe Amalia , Ramon Villa Jose , Argente Jesus , Martos-Moreno Gabriel A.

Introduction: Cystic fibrosis related diabetes (CFRD) is associated with a poorer nutritional status, respiratory function and an increase in mortality rate. Screening is recommended from age 10; however, prediabetic conditions are diagnosed earlier.Objectives: 1) To characterize the degree of carbohydrate metabolism impairment (CMI) in 50 CF patients. 2) To explore the association with clinical parameters as eventual predictors of these conditions.<...

hrp0086p2-p182 | Bone &amp; Mineral Metabolism P2 | ESPE2016

Primary Hyperparathyroidism- A Cause of Metabolic Syndrome in Children?

Ioana Arhire Amalia , Pavel Madalina , Florea Suzana , Miron Adrian , Gabriela Barbu Carmen

Introduction: We report a case of a 15 year old male with metabolic syndrome due to primary hyperparathyroidism.Case report: A 15 year old male was admitted in our department for the evaluation and management of obesity. His medical history revealed a progressive weight gain in the last 3 years in an emotional familial context. Clinical features: Obesity: Z-score of +2.32 DS, a height of 1.80 m and a weight of 108 kg, with a BMI of 33.3...