Background: The GH/IGF1 axis has important roles in growth, metabolism, lipid profile and body composition. GH receptor disrupted mice (GHRKO mice) are resistant to the action of GH, thereby, GHRKO mice are dwarf, hypoinsulinemic, hypoglycemic and obese. Consumption of a high fat diet (HFD) induces inflammatory processes in a multitude of peripheral tissues, including hypothalamus.
Objective and hypotheses: Our aim was to evaluate the effect of HFD intake on hypothalamic inflammation in the absence of GH signalling.
Method: Male C57BL/6J WT and GHRKO mice, 30 days of age were feed with standard chow (normal diet; ND) or HFD (60% fat) during 50 days and were then sacrificed. Levels of cytokines were measured in the hypothalamus by multiplex assay.
Results: The absence of GH signalling in young mice has an impact on lipid metabolism that leads to an increase in circulating NEFA levels (WTND: 1.1±0.08; WTHF: 0.9±0.05; KOND: 1.8±0.16; KOHF: 1.3±0.01. P<0.01) that could induce hypothalamic inflammation. While HFD had no effect in WT mice, it decreased serum NEFA levels and hypothalamic cytokines in GHRKO mice (Table 1).
|ANOVA: *P<0.01; vs WT ND; †P<0.01 vs KO ND; ‡P<0.01 vs WT HFD. n=56/group.|
Conclusion: As increased NEFA levels have been associated with hypothalamic inflammation, the decreased hypothalamic cytokine levels could be due to the decline in NEFA induced by HFD in GHRKO mice.
20 - 22 Sep 2014
European Society for Paediatric Endocrinology