ESPE Abstracts (2014) 82 P-D-2-2-426

The Level and Conformation of Blood Plasma Carotenoids in GH Deficient Children After 1 Year of GH Therapy

Maria Faassena, Maria Pankratovaa, Tatyana Shiryaevaa, Elena Nagaevaa, Valentina Peterkovaa, Svetlana Kovalenkob, Adil Baizhumanovb, Evgenia Parshinab, Alexander Yusipovichb & Georgy Maksimovb

aEndocrinology Research Centre, Moscow, Russia; bMoscow State University, Moscow, Russia

Background: Epidemiologic studies have shown strong associations between high carotenoids levels and reduced risk of developing various forms of cancer, cardiovascular system diseases, etc.

Objective and hypotheses: Carotenoids are hydrophobic substances and are contained in blood in lipoprotein particles. Their conformation depends on their molecular environment. By evaluating the conformation of carotenoids we can obtain various information about the lipoprotein structure. The aim was to examine the total level of carotenoids and their conformation during GH treatment in prepubertal children with GHD and to elucidate their relationship to oxidative stress development.

Method: 11 treatment-naïve prepubertal children with GHD were included in the study. The conformation and total concentration of carotinoids were examined using Raman spectroscopy, their concentration – by calculating the area of Raman spectrum peak, the conformation was assessed as Raman spectrum select band ratios. The blood antioxidant system was examined using the activity of superoxide dismutase and catalase, thiobarbituric acid reactive substances and ceruloplasmin levels and the total antioxidant capacity of plasma.

Results: The concentration of plasma carotenoids before treatment did not differ from data after treatment. The mean values of band ratios I1525/I1160 and I1160/I1008 before and after treatment did not differ. However, the mean value of ratio I1525/I1008 after treatment decreased by 60%. The plasma carotenoid concentration didn’t change significantly.

Conclusion: The lack of change in total carotenoids levels, the same as a antioxidant parameters, during treatment in children with GHD probably indicates the absence of oxidative stress development and the lack of deterioration during treatment correspondingly. The decreasing of the band ratio at I1525/I1008 was probably caused by changing of characteristics of the C-CH3 bonds in carotenoids as a result of alterations in lipoproteins structure.