ESPE Abstracts (2015) 84 P-1-148

The Analysis of Occurrence the Zinc Transporter Antibodies ZnT8 in Children with Graves' Disease and Hashimoto's Thyroiditis

Artur Bossowskia, Hanna Borysewicz-Sanczyka, Anna Bossowskab, Mieczyslaw Szaleckic,h, Anna Kucharskad, Beata Pyrzakd, Mieczyslaw Walczake, Elzbieta Petriczkoe, Katarzyna Zioraf, Maria Del Pilar Larosag, Shu Cheng, Michael Powellg, Jadwiga Furmaniakg & Bernard Rees Smithg


aEndocrinology and Diabetes with a Cardiology Unit, Department of Pediatrics, Medical University in Bialystok, Bialystok, Poland, bDivision of Cardiology, Internal Affairs and Administration Ministry Hospital in Bialystok, Bialystok, Poland, cClinic of Endocrinology and Diabetology, Children’s Memorial Health Institute, Warsaw, Poland, dDepartment of Pediatrics and Endocrinology, Medical University in Warsaw, Warsaw, Poland, eDepartment of Pediatrics, Endocrinology, Diabetology, Metabolic Diseases and Cardiology of the Developmental Age, Pomeranian Medical University, Szczecin, Poland, fDepartment of Pediatrics, Silesian Medical University, Zabrze, Poland, gFIRS Laboratories, RSR Ltd, Parc Ty Glas, Llanishen, Cardiff CF14 5DU, Cardiff, UK, hInstitute of Health Sciences, UJK, Kielce, Poland


Introduction: Recent studies have revealed the presence of zinc and the expression of zinc transporter (ZnT) family members in most endocrine cell types. Moreover it was demonstrated that the ZnT family plays an important role in the synthesis and secretion of many hormones like insulin. We studied the prevalence of ZnT8 Ab in patients with autoimmune thyroid diseases (AITD).

Material and methods: The study was performed in the group consisting of 20 Graves’ disease (GD) patients (mean age, 17.8±1.4 years), 44 Hashimoto’s thyroiditis (HT) patients (mean age, 13.8±3.5 years) and 57 controls (mean age, 13.1±3.5 years). Patients were recruited from few endocrine centers.GAD,IA-2,IAA,ZnT8,21-OH and AChR antibody concentrations were evaluated in the sera using RSR kits.

Results: In our study, ZnT8Ab were found in four patients (20%) with GD while three patients (15%) were positive for GADAb, one patient (5%) was positive for IAA and one patient (5%) was positive for IA-2Ab. Of these, one GD patient was positive for all four diabetes associated antibodies. In the case of HT patients, 4 (9%) were positive for ZnT8Ab, while three patients (7%) were positive for GADAb, 2 (4.5%) were positive for IA-2Ab and 1 (2.3%) was positive for IAAAb. Of these, one HT patient had three diabetes associated antibodies (ZnT8,GAD & IA-2Abs) and one had two diabetes associated antibodies (GADAb and IAA). Out of 57 controls studied, 2 (3.5%) controls were positive for ZnT8 Ab, one (1.8%) was positive for GADAb and none of them was positive for IA-2Ab or IAA. Furthermore, one GD patient (5%) and two HT patients (4.5%) were positive for 21-OHAb only.None of the patients with AITD and controls studied was positive for AChRAb.

Conclusion: In conclusion, these results suggest that the presence of ZnT8Ab can be associated with other autoimmune diseases other than T1DM in particular Graves’ disease and Hashimoto’s thyroiditis.