ESPE Abstracts (2015) 84 P-2-297

GH Promotes mRNA Expression and Secretion of Progranulin in 3T3-L1 Cells

Guo Song, Chen Hongshan, Zhang Jun, Du Minlian, Ma Huamei, Li Yanhong, Chen Qiuli & Peng Yangshui

Hospital of Sun-yat Sen University, Guangzhou, China

Background: Recently, progranulin (PGRN) was a novel adipokine which is a key adipokine insulin resistance in adipose tissue. While GH was closely related to glucose metabolism and insulin resistance.

Objective and hypotheses: We suspected that there maybe some positive relationship between GH and PGRN. Our study was to detect expression and regulation of PGRN in mouse 3T3-L1 cells follow the treatment with GH.

Method: The mRNA was measured by quantitative PCR and the protein was tested by Western blot in mouse 3T3-L1 cells follows a series of concentrations and treating time of GH.

Results: We find that both Western blot analyses and quantitative PCR showed that 500 ng/ml GH increases the expression of PGRN in a quick reaction (0.5–2 h), and decreases since 4 h, while 5 ng/ml GH promotes the expression of PGRN but without change with time.

Conclusion: Our results show that GH regulated the expression of PGRN in a time and concentration dependent manner.

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