Aım: İn this prospective planned study, it was aimed to study relationship between vitamin D level and vitamin D receptor gene polimorphism in patients with type l DM aged 018 years old.
Methods and materials: We enrolled total 165 children in which 101 new and old diagnosed to WHO criterias TIDM patients from 018 years old and 64 chıldren as control group who were matched with gender and age. İn all cases serum Ca, P, PTH, ALP, Mg, albumine, 25-OH vitamin D and vitamin D receptor gne polimorphisms (Bsml, Apal, Taql, Fokl and Cdx-2) and also in patients insulin, C-peptide, HbA1c levels were studied. DNA izolations were obtained from patients and control groups peripheral blood samples for VDR gene polimorphism. Fındıngs: 43 (%42.6) of TIDM patients were female, 58 (%57,4) male. 30 (%46.9) of control group were female, 34 (%56.1). Mean age in patients was 11.69±3.70 in control group was 10.7±6.14 years. Mean vitamin D level in TIDM patients was 14.32±6.14 ng/ml, in control group 14.17±14.42 ng/ml and no statistical difference was found in vitamin D level between two groups (P>0.05). Vitamin D level in %82.7 male TIDM patients was low. %55.1 of these patients were vitamin D insufficient, %27.5 vitamin D deficient. Vitamin D level was low in %81.3 of female patients with TIDM %39.5 of these patients were vitamin D insufficient and % 41.8 vitamin D deficient. No significant difference was found in low level of vitamin D between male and female patients with TIDM (P>0.05). Altough there was no statistical significant difference in VDR gene polimorphisms with neither vitamin D quantative level nor categorical arrangement (deficient,insufficient and normal), in correlation analyses, significant positive correlation was confirmed between Fokl polimorphism and age, body weight and BMI level in control group. For Fokl polimorphism when adjustment was done to values above to patient and control groups, there was statistical significant difference in Fokl polimorphism in patient and control groups (P>0.05). Also in correlation analyses while positive correlation was found between Fokl-Bsml and Bsml-Apal, negative correlation was found between Bsml-Taql and Apal-Taql. When patients with TIDM compared with control group no statistical significant difference was found in vitamin D gene polimorphism (Bsml, Taql, Apal, Fokl and Cdx-2) (P>0.05).
Results: İn our study while there wasnt any relationship between patients with T1DM vitamin D receptor gene polimorphisms (Bsml, Taql, Apal, Fokl and Cdx-2) it seemed as if polimorphism in Bsml or Apal and polimorphism in Taql effected each other adversly. Also while no significant difference was found in serum vitamin D level in both groups, no statistical significant correlation was determined between metabolic control and serum vitamin D level in patient group (P>0.05). Our study andn other contraversary studies results show that further clinical studies which will be done with same population and larger series are needed to assess anterograde vitamin D effect on TIDM development and VDR gene polimorphism.
Funding: Proje 2014-TF-U059.
01 - 03 Oct 2015
European Society for Paediatric Endocrinology