ESPE Abstracts (2015) 84 P-3-937

The Blood Oxidant System and Insulin Resistance in Girls with Turner Syndrome after 1 Year of GH Therapy

Maria Pankratovaa, Maria Faassena, Tatyana Shiryaevaa, Elena Nagaevaa, Valentina Peterkovaa, Adil Baizhumanovb, Alexander Yusipovichb, Alexander Cherkashinb & Georgy Maksimovb

aDepartment of Pediatric Endocrinology, Endocrinology Research Center, Moscow, Russia; bBiophysics Department, Faculty of Biology, Moscow State University, Moscow, Russia

Background: The effects of recombinant GH therapy on development of oxidative stress and insulin resistance in girls with Turner syndrome (TS) were observed.

Objective and hypotheses: The aim of this study is to examine the longitudinal relationships of oxidative stress markers with the development of insulin resistance during GH treatment in girls with TS.

Method: Ten prepubertal girls (aged 12–14 years; median 13.0 years) with TS were included in the study. All of them have not been treated with GH before. Blood antioxidant system was examined using activity of superoxide dismutase and catalase, thiobarbituric acid reactive substances (TBARS), ceruloplasmin level and total antioxidant capacity (TAC) of plasma. Levels of lipids, glucose, insulin, HbA1c and IGF-1 were measured in blood plasma before and after 1 year of GH treatment (0.05 mg/kg per day). The insulin resistance assessed using the homeostasis model assessment of insulin resistance (HOMA-IR).

Results: The concentration of plasma insulin level in girls with TS after 1 year treatment GH was significantly higher than before (7.2±3.4 vs 14.5±4.9 mU/l, P=0.003). The values of HOMA-IR in girls with TS after treatment GH was significantly higher than before (1.6±0.8 vs 3.2±1.22, P=0.008). Before treatment value of HOMA-IR was less than 3.2 (upper reference limit) from all patients, after treatment it was more than 3.2 in five of ten patients (max value of HOMA-IR was 4.7). Also after treatment the value of TBARS was significantly greater (about 40%) and the catalase activity was significantly lower (about 30%) than before treatment.

Conclusion: So GH treatment in girls with TS after 1 year GH treatment promoted insulin resistance accompanied by the development of mild form of oxidative stress.