ESPE Abstracts (2016) 86 P-P1-367

ESPE2016 Poster Presentations Gonads & DSD P1 (48 abstracts)

Ovarian Reserve Assessment in Girls and Women after Hematopoietic Stem Cell Transplantation Treatment Underwent in Childhood

Anna Wedrychowicz , Joanna Wojtys & Jerzy B. Starzyk

Department of Pediatric and Adolescent Endocrinology, Pediatric Institute, Medical College, Jagiellonian University, Cracow, Poland

Background: Hypogonadism is one of the most frequent endocrine complication after hematopoietic stem cell transplantation (HSCT). In some patients hypogonadism could be transient, but very often coexists with prematury ovarian failure. Classical methods used in the diagnostics of hypogonadism have limitations for the prognosis of ovarian reserve.

Objective: The aim of the study was to assess ovarian reserve in patients after HSCT using evaluation of anti-Muellerian hormone (AMH) with comparison to classical hormonal methods.

Methods: Twenty-eight patients, median age 15.8 years, after allogeneic (19) and autologic (9) HSCT and 28 healthy age-matched controls were included in the study. Fasting blood samples for the measurement of FSH, LH, estrogens, PRL, SHBG, TSH, fT4, AMH, and Inhibin-B, if possibly in 3–5th day of the menstruation cycle, were taken. Hormones were measured by immunochemistry. Statistical analysis was performed using t-Student test.

Results: AMH and inhibin-B levels were significantly lower (P<0.001) and FSH and LH levels were significantly higher in patients after HSCT than in controls (P<0.01). In 20/28 patients AMH level was below 0.08 ng/ml as postmenopausal, only 2/28 have normal AMH values. Hypergonadotropic hypogonadism was observed in 21/28 patients, 7/28 patients have normal values of sex hormones (3/7 prepubertal). Patients with normal AMH, have normal other hormones and normal menstruation cycles (severe aplastic anemia). Six patients (Fanconi anemia 3, solid tumors 3) with AMH levels 0.08–1 ng/ml presented with prepubertal status with low FSH (3) spontaneous puberty (1) and normal menstruations (2). Their inhibin B level was within normal ranges.

Conclusion: Patients after HSCT have impaired ovarian reserve. The ovarian reserve is mostly related to the conditioning therapy before HSCT. AMH as well as Inhibin-B and FSH are specific and good markers for the assessment of ovarian function. AMH seems to be more sensitive test than other markers in the evaluation of ovarian reserve after HSCT.

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