ESPE Abstracts (2018) 89 P-P1-039

3-epi-25 Serum 25-hydroxyvitamin D3 Concentrations in Chilean Children between 5 and 8 Years

Monica Arancibia, Cristian Seiltgens, Helena Poggi, Fidel Allende, Sandra Solari, Soledad Peredo, Claudia Trincado, Hernan Garcia, Rosario Moore, Ivonne Dàpremont, Daniela Andrade, Sofia Sifaqui, Jt Ossa, Carmen Campino, Cristian Carvajal, Carlos Fardella, Rene Baudrand, Ximena Sanchez & Alejandro Martinez-Aguayo

Pontificia Universidad Catolica De Chile, Santiago, Chile

Background: The C3 epimer of 25-hydroxi-vitamin D3 (Epi25OHD3) is present in the pediatric and adult population and varies according to age. If it measurement is clinically relevant and should be considered to classify Vitamin D status is still unknown.

Objective: To measure 25OHD3, 25-hydroxy-Vitamin D2 (25OHD2) and Epi25OHD3 and to compare them with PTH and calcemia.

Method: Subjects: Children between 5 and 8 years of age born very preterm (VPT: <32 weeks of gestation) and term (≥38 weeks of gestation).

Exclusion criteria: SGA (weight ≤−2 SD), multiple pregnancy, chronic and acute disease and use of oral corticosteroids.

Measurements: 25OHD2, 25OHD3 and Epi25OHD3 by mass spectrometry (LC-MS/MS). 25OHD3 status (ng/mL): deficient <20, insufficient 20-30, sufficient ≥ 30. Total 25OHD3: 25OHD3+ Epi25OHD3. Statistical test: U-Mann Whitney.

Results: Seventy seven (83%) of a total of 93 subjects (45% female) had detectable Epi25OHD3 concentrations and only 2 subjects 25OHD2. An association was found between Epi25OHD3 and 25OHD3 (r=0.57, P <0.0001), calcemia (r=0.84, P=0.005) and PTH (r=0.18, P <0.0001). Gestational age was correlated to Epi25OHD3 (r=0.265, P=0.011) and 25OHD3 (r=0.229, P=0.036). There were differences in the percentage of total Epi25OHD3/25OHD3 between very premature and term subjects (P=0.04). When adding the concentrations of Epi25OHD3 to those of 25OHD3 (25OHD3 total) the percentage of Epi25OHD3/total 25OHD3 in VPT and term children was different (9.7% and 10.6% respectively). Vitamin D status changed. When categorizing Vitamin D status using total 25OHD3, the percentage of children with deficiency dropped from 19.4% to 12.9%, with insufficient from 54.8% to 47%, those with a sufficient status raised from 25.8% to 39.8%.

Conclusion: At school age, term as well as very preterm children have detectable levels of Epi25OHD3. It is necessary to establish the physiological role of Epi25OHD3, since when considered to establish Vitamin D status, it changed in an important proportion of children. Including its measurement in routinely used immunoassays and their harmonization in this regard, could be relevant in the classification of Vitamin D status.

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