Background: Recent research has demonstrated earlier testicular and pubertal development in Western boys. New ultrasound-based references of testicular growth in Norwegian boys are now available. Population specific references for FSH, LH and sex steroid hormones have not been previously available in Norwegian children and adolescents.
Objective and hypotheses: We aimed to provide an elaborate description of pubertal development in a sample of contemporary Norwegian boys, based on clinical evaluation, ultrasound determined testicular volume and hormonal levels.
Method: As part of the cross-sectional Bergen Growth Study 2, we examined 451 boys aged 6-16 years. All boys were examined during school hours which included assessment of Tanner P-stage, ultrasound imaging of the testicular length, height and width (converted to volume), and collection of blood samples. Total testosterone levels were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and peptide hormones FSH, LH and SHBG were measured on Immulite 2000. Reference intervals were estimated with the GAMLSS framework within R.
Results: Reference intervals for the hormonal values based on age, testicular volume and Tanner P-stages are presented.
Conclusion: Novel and objective references for key pubertal hormones and ultrasound-based testicular volume are available to complement traditional Tanner staging for clinical assessments of individual children during the pubertal transition.
27 Sep 2018 - 29 Sep 2018