ESPE Abstracts (2019) 92 P1-255

Pituitary, Neuroendocrinology and Puberty (1)

Effects of 5-Hydroxymethylfurfural on Pubertal Development of Wistar Rats

Selin Elmaogullari1, Elcin Kadan2, Elvan Anadol3, Ayris Gokceoglu4, Semra Cetinkaya1, Gul Fatma Yarim4, Seyit Ahmet Ucakturk5, Zehra Aycan1


1 Dr. Sami Ulus Children Training and Research Hospital, Department of Pediatric Endocrinology, Ankara, Turkey. 2Gulhane Training and Research Hospital, Department of Pathology, Ankara, Turkey. 3Gazi University, Laboratory Animal Breeding and Experimental Researches Center, Ankara, Turkey. 4Ondokuz Mayis University, Faculty of Veterinary Medicine, Department of Biochemistry, Samsun, Turkey. 5Ankara Children Hematology and Oncology Training and Research Hospital, Department of Pediatric Endocrinology, Ankara, Turkey

Introduction: 5-Hydroxymethylfurfural (HMF) is formed when sugars like glucose and fructose are heated in the presence of amino acids. HMF is naturally present in many foods and we are exposed to HMF in daily life. There are conflicting data on potential genotoxic, mutagenic, carcinogenic, DNA-damaging, organotoxic and enzyme inhibitory effects of HMF and its metabolites. We aimed to investigate toxic effects of HMF on reproductive system in peripubertal rats.

Methods: In the study, 24 immature Wistar rats were divided into control and HMF groups fed 750 mg/kg/day and 1500 mg/kg/day for 3 weeks from postnatal day 21. They were controlled for vaginal opening (VO) daily and necropsied on postnatal day 44. Blood samples were collected with cardiac puncture on termination day. Follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), progesterone (P) and anti-Müllerian hormone (AMH) levels in blood serum were measured using rat-specific enzyme-linked immunosorbent assay kits. Hormone levels, reproductive organ weights and ovarian follicle counts were compared.

Results: High dose HMF group had earlier VO with higher LH and E2 levels. High dose HMF group also had increased number of secondary atrophic follicles and decreased AMH levels.

Conclusion: These results indicate that peripubertal exposure to HMF in high doses result in precocious puberty and decreased ovarian reserve in Wistar rats.

Volume 92

58th Annual ESPE meeting

Vienna, Austria
19 Sep 2019 - 21 Sep 2019

European Society for Paediatric Endocrinology 

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