Introduction: 5-Hydroxymethylfurfural (HMF) is formed when sugars like glucose and fructose are heated in the presence of amino acids. HMF is naturally present in many foods and we are exposed to HMF in daily life. There are conflicting data on potential genotoxic, mutagenic, carcinogenic, DNA-damaging, organotoxic and enzyme inhibitory effects of HMF and its metabolites. We aimed to investigate toxic effects of HMF on reproductive system in peripubertal rats.
Methods: In the study, 24 immature Wistar rats were divided into control and HMF groups fed 750 mg/kg/day and 1500 mg/kg/day for 3 weeks from postnatal day 21. They were controlled for vaginal opening (VO) daily and necropsied on postnatal day 44. Blood samples were collected with cardiac puncture on termination day. Follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), progesterone (P) and anti-Müllerian hormone (AMH) levels in blood serum were measured using rat-specific enzyme-linked immunosorbent assay kits. Hormone levels, reproductive organ weights and ovarian follicle counts were compared.
Results: High dose HMF group had earlier VO with higher LH and E2 levels. High dose HMF group also had increased number of secondary atrophic follicles and decreased AMH levels.
Conclusion: These results indicate that peripubertal exposure to HMF in high doses result in precocious puberty and decreased ovarian reserve in Wistar rats.
19 - 21 Sep 2019
European Society for Paediatric Endocrinology