ESPE Abstracts (2019) 92 RFC1.2

Diabetes and Insulin Session 1

Gabra5 Contributes to Sexual Dimorphism in POMC Neural Activity and Glucose Metabolism

Zhou Pei


Children's Hospital of Fudan University, shanghai, China

Objectives: To test whether Gabra5 in POMC neurons regulates energy balance and glucose metabolism in sexually dimorphic fashion.

Methods: POMC-Cre mice received stereotaxic injections of AAV-FLEX-scCAS9 and AAV-FLEX-Gabra5-sgRNA-tdTOMATO into ARH to generate pomc-Gabra5 KO mice. Both male and female mice were fed on regular chow diet or HFD. Food intake, body weight and anal temperature were measured every 4 days. Fat/lean mass were measured by QMR. Glucose tolerance test and insulin tolerance test were done before and after HFD feeding. Glucose clamp was also done after 4 weeks HFD feeding.

Results: Neither on chow diet nor HFD, no significant difference was found in food intake, body weight and anal temperature in males and females compared with their controls. Female Gabra5-KO mice on HFD showed impaired insulin tolerance. While male Gabra5-KO mice on HFD showed improved insulin tolerance.

Conclusions: Specific deletion of Gabra5 in POMC neuron doesn't relate to energy expenditure neither in males nor females. However, pomc-Gabra5 KO could improve glucose tolerance only in male mice, while female mice showed opposite result. These results provide evidence for sex differences in glucose metabolism.

Volume 92

58th Annual ESPE meeting

Vienna, Austria
19 Sep 2019 - 21 Sep 2019

European Society for Paediatric Endocrinology 

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