ESPE2022 Poster Category 1 Pituitary, Neuroendocrinology and Puberty (77 abstracts)
Simultaneous quantification of 51 steroids in pediatric plasma and urine samples by LC–MS/MS
Xiaoyu Wu 1 , Yao Chen 2 , Xiaoqing Li 3,4 , Peng Xue 3,5 , Jingyi Tang 3,4 , Xiaodan Yu 6 , Liang Zheng 1 , Xiumin Wang 7 & Shijian Liu 8,4
1Pediatric Translational Institute, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; 2Department of Endocrinology, Genetics and Metabolism, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiao Tong University, Shaghai, China; 3Department of Clinical Epidemiology and Biostatistics, Children Health Advocacy Institute, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; 4School of Public Health, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; 5School of Public Health, School of Medicine, Shanghai Jiao Tong University, Shangai, China; 6Department of Developmental and Behavioral Pediatrics, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; 7Department of Endocrinology, Genetics and Metabolism, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; 8Department of Clinical Epidemiology and Biostatistics, Children Health Advocacy Institute, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanhai, China
Quantitative analysis of endogenous and exogenous steroid hormones is an important step for our understanding of the causes of endocrine disorders in pediatric. In this study, we developed a method to simultaneously quantify endogenous and exogenous steroids in pediatric urine and plasma. Ultra-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to simultaneously quantify steroid hormones in children plasma and urine. Under the optimum conditions, the method was achieved with a BEH RP18 column within 18 min. The method had excellent linearity (R2.0.990) and a low quantification limit of 0.01~1 ng/mL. And the precision of most steroid hormones is less than 20%, and the recoveries range from 80% to 140%. The results show that the method has good stability and sensitivity, and can be successfully used for the analysis of steroids in human plasma and urine. Compared with the control group, the endogenous steroids (corticosterone, cortisone, cortisol, etc.) in the experimental group significantly decreased and the fold change was 1.5-2.1-fold, while the exogenous steroids (Prednisone acetate and triamcinolone diacetate) increased by 1.84 and 1.86-folds. The present findings demonstrate that the LC-MS/MS method enables rapid and robust quantification of multiple steroid hormones in plasma and urine samples.
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