ESPE Abstracts

Objective: To explore the correlation of sex hormone-binding globulin (SHBG) and Total Testosterone (TT) with the development of Metabolic syndrome (MetS) in obese boys. To explore the relationship between components of MetS and TT levels in boys.

Methods: A total of 439 boys aged 6-18 years old from April 2020 to February 2023, include boys who visited the Department of Endocrinology, Genetics and Metabolism in Jiangxi Province Children's Hospital and healthy boys who underwent physical examination in our hospital were collected. According to the diagnostic guidelines for metabolic syndrome, dividing those boys into Control (CON) group (76); obesity (OB) group (249); MetS group (114). Collecting data and using spss26.0 software to analyze and compare the differences of metabolic indices and TT level between three groups. And analyzing the relationship of TT level among those group. Eight boys randomly from each three group were selected as subjects for targeted proteomic analysis, screening differential protein for functional analysis and protein interaction network analysis.

Result: 1. Clinical data analysis: The CON group, OB group and MetS group were pairwise compared, and age, puberty and HbA1c were no statistically significant differences (P > 0.05) in three pairwise comparisons, respectively. Compared with CON and OB groups, BMI, SBP, DBP, LDL-C, non-HDL-C, TG, TC, FBG, FINS, HOMA-IR, ALT and AST levels were higher in MetS group, and HDL-C, FAA and TT levels were lower(P < 0.05). Analyzing the correlation between TT and other variables in the sample showed that TT level was independently correlated with puberty, TC and age (P<0.05). After adjusting for confounding factors of puberty, TC and age, Compared with CON and OB groups, TT levels were lower in MetS group, OR values and 95%CI were 1.012 (1.005-1.020) and 1.009 (1.003-1.016). 2. Proteomic analysis: The CON group, OB group and MetS group were pairwise compared all showed SHBG is significantly down-regulated differential proteins;proteins associated with SHBG are IGF1, IGFBP1 and CRP.

Conclusion: 1. BMI, SBP, DBP, LDL-C, non-HDL-C, TG, TC, FBG, FINS, HOMA-IR, ALT and AST of MetS boys were higher than those of healthy boys and obese boys; HDL-C, FAA and TT were lower than healthy boys and obese boys. 2. TT level was independently correlated with puberty, TC and age; Low TT can be an independent risk factor for the development of metabolic syndrome in boys. 3. Decreased SHBG level is an independent risk factor for MetS in boys.