ESPE Abstracts

Objective: To investigate the role of serum anti-mullerian hormone (AMH) and inhibin-B (INHB) in central precocious puberty precocious puberty (CPP) and early and fast puberty(EFP).

Methods:90 girls with CPP, EFP and premature thelarche (PT) were enrolled in our hospital from January 2021 to December 2022, and 45 girls without healthy development were enrolled. General data, sex hormones, AMH, INHB levels and gonad ultrasound were collected. The data of each group were compared, and the diagnostic value of AMH and INHB in predicting CPP girls and their early warning value in EFP girls were judged by receiver operating characteristic curve, and the correlation of each index was analyzed by Spearman correlation analysis. Girls diagnosed with CPP or EFP at the same time were selected to receive GnRHa treatment. AMH, INHB, sex hormone detection, uterine volume (UV) and mean ovarian volume (M-OV) were evaluated at the 6th and 12th month of treatment. The changes of each index before and after treatment were compared to explore whether AMH and INHB can be used to evaluate the therapeutic effect of GnRHa. Results AMH in CPP group was higher than that in PT group, while INHB in CPP group was higher than that in non-CPP group (PT group and healthy group). The sensitivity and specificity of INHB in the diagnosis of CPP were 0.815 and 0.611, respectively, while the area under ROC curve of AMH in the diagnosis of CPP was less than 0.5. AMH was negatively correlated with LHb, FSHb, LHp and FSHp levels. The sensitivity and specificity of INHB early warning EFP were 0.887 and 0.606, respectively. The sensitivity and specificity of INHB combined with UV early warning EFP were 0.962 and 0.576, respectively. INHB was positively correlated with UV, LHb, FSHb and LHb/FSHb levels in EFP. After 6 and 12 months of GnRHa treatment, INHB decreased significantly in both CPP and EFP, and the decrease rate of INHB was positively correlated with the decrease rate of LHp and FSHp. The change of AMH level before and after treatment was not statistically significant.

Conclusions: This study verified the diagnostic value of INHB in CPP girls. This study suggests that AMH level can be used as a potential indicator to distinguish central precocious puberty from early breast development. INHB level combined with UV detection is significant for early warning of EFP girls in clinic;INHB has important reference value in evaluating the efficacy of GnRHa in CPP and EFP girls.