ESPE Abstracts

Objectives: To compare the levels of estradiol (E2) in girls measured by liquid chromatography tandem-mass spectrometry (LC-MS/MS) and chemiluminescence immunoassay (CLIA), and to evaluate the correlations between E2 levels measured by the two methods and bone age, uterine length, and uterine volume. To explore the difference, consistency, and accuracy of LC-MS/MS and CLIA in determining E2 in girls with idiopathic central precocious puberty (ICPP), premature thelarche (PT), and normal puberty, and to guide clinical diagnosis and treatment.

Methods: From July 2022 to December 2022, 133 newly diagnosed girls aged 7-9 were selected from the Department of Endocrine Genetics and Metabolism, Children's Hospital Affiliated to Soochow University. There were 80 girls in the ICPP group (44 in the Tanner II stage in the ICPP group, 36 in the Tanner III location in the ICPP group), and 53 in the PT group. A total of 40 healthy girls aged 9-10 years in the Tanner III group of ordinary puberty, The age, gonadotropin-releasing hormone (GnRH) stimulate test, bone age, and uterus and ovary ultrasound results were collected, and CLIA and LC-MS/MS methods detected essential serum E2. The serum basal E2 levels of girls in the PT group, ICPP group Tanner II, ICPP group Tanner III and standard pubertal Tanner III group were compared by the CLIA method and LC-MS/MS method. SPSS 26.0 software was used for statistical analysis of E2 data.

Results and Conclusions: 1. The E2 level detected by LC-MS/MS method differs from that seen by the CLIA method. LC-MS/MS method has higher sensitivity and lower E2 class. The sample size required for detection is smaller, and LC-MS/MS method can quantitatively detect E2 in a lower concentration range. 2. The stability of the LC-MS/MS method for detecting E2 is higher than that of the CLIA method and has higher specificity. 3. The consistency between E2 detected by LC-MS/MS and the CLIA method could not be better. The detection method and its reference range should be fully understood when evaluating the E2 level in the clinic. 4. The LC-MS/MS method's accuracy for detecting E2 is better than that of the CLIA method, especially in PT girls with low E2 levels. The accuracy of the two methods for detecting E2 was similar in the ICPP group of Tanner III girls with high E2 levels.