ESPE2024 Poster Category 3 Fat, Metabolism and Obesity (35 abstracts)
Exploring leptin dysfunction in severe early-onset obesity: implications for pediatric health – a preliminary report
Sabina Galiniak 1 , Marek Biesiadecki 1 , Rafał Podgórski 1 , Natalia Dąbek 2 , Ewa Gramatyka-Drążek 1 , Ewelina Gaweł 1 , Michael B. Ranke 3 , Bertram Flehmig 4 , Martin Wabitsch 5 , Stephanie Brandt 5 , Elżbieta Petriczko 6 , Małgorzata Wójcik 7 , Ewa Małecka-Tendera 8 , Mirosław Bik-Multanowski 9 , Agnieszka Zachurzok 10 & Artur Mazur 1
1Institute of Medical Sciences, Medical College, Rzeszów University, Rzeszów, Poland. 2Institute of Medical Sciences, Medical College, Rzeszow University, Rzeszów, Poland. 3University Children's Hospital, Tübingen, Germany. 4Mediagnost GmbH, Reutlingen, Germany. 5Center for Rare Endocrine Diseases, Division of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, Ulm, Germany. 6Department of Pediatrics, Endocrinology, Diabetology, Metabolic Disorders and Cardiology of Developmental Age, Pomeranian Medical University, Szczecin, Poland. 7Department of Pediatric and Adolescent Endocrinology, Pediatric Institute, Jagiellonian University Medical College, Cracow, Poland. 8Department of Pediatrics and Pediatric Endocrinology, Medical University of Silesia, School of Medicine in Katowice, Katowice, Poland. 9Department of Medical Genetics, Faculty of Medicine, Jagiellonian University Medical College, Cracow, Poland. 10Department of Pediatrics, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Zabrze, Poland
Severe early-onset obesity (SEOO) increases the likelihood of obesity persisting into adulthood. Leptin (LEP), a hormone primarily produced in adipose tissue, plays a crucial role in regulating energy balance. When assessing young children with SEOO, it is important to consider abnormalities in the leptin-melanocortin pathway. Our study aim ed to identify children with SEOO who may have LEP deficiency or biologically inactive leptin by analyzing leptin and biologically active leptin (bioLEP) concentrations in serum, and to examine the associations between LEP, bioLEP levels and anthropometric parameters in these children. The study comprised 400 children, aged 12.5±6.4 years, diagnosed with SEOO (184 boys, 46%), recruited from four Polish study centers (Zabrze, Cracow, Rzeszów, and Szczecin) between August 2023 and March 2024. Children meeting the inclusion criteria of having a body mass index (BMI) >25 kg/m² before the age of 6, whose parents provided informed consent, were enrolled. Measurements of weight, height, and bioimpedance were recorded, along with taking of fasting serum blood samples. BMI and BMI z-scores (based on WHO standards) were calculated. Serum levels of total LEP and bioLEP were determined using ELISA kits from Mediagnost, Reutlingen, Germany. BioLEP/LEP ratios were calculated. The mean BMI for the entire group was 37.32±7.2 kg/m², with a BMI z-score of 3.18±0.82. Overall, the LEP concentration was 85.16±29.7 ng/mL, and bioLEP was 85.32±27.46 ng/mL. For girls, LEP concentration was 75.7±30.73 ng/mL and bioLEP was 77.03±28.48 ng/mL, while for boys, it was respectively 84.02±26.7 ng/mL (P <0.001) and 82.02±24.2 ng/mL (P <0.001). Individual bioLEP/LEP ratios were approximately 1.00 (whole group: 1±0.14, girls: 1.02±0.14, boys: 0.97±0.13, P = 0.069). Higher age weakly correlated with elevated LEP (r =0.14, P <0.01) and bioLEP (r =0.14, P <0.01). Additionally, LEP and bioLEP levels were positively correlated with BMI values (r =0.45, P <0.001 and r =0.46, P <0.001, respectively). LEP and bioLEP levels were positively correlate with fat mass (r =0.45, P <0.001 and r =0.47, P <0.001, respectively). Collectively, our findings underscore the multifaceted role of leptin in the pathogenesis of SEOO and emphasize the importance of elucidating leptin-mediated mechanisms underlying obesity onset and persistence, with implications for developing targeted therapeutic interventions to mitigate obesity-related disorders. Funding: This research was funded by National Science Center, Poland (2021/41/B/NZ5/01676).
Article tools
My recent searches
My recently viewed abstracts
Authors
ESPE Abstracts
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more