ESPE Abstracts

Objective: Serum Anti-Mullerian Hormone (AMH) and Inhibin-B (INHB) levels were assessed in patients with Turner Syndrome (TS) of different karyotypes, and ovarian reserve function was evaluated by AMH and INHB in patients with TS.

Methods: From January 2021 to January 2024, 52 patients diagnosed with TS aged 4-17 years were diagnosed in the Department of Endocrinology, Genetics and Metabolism of Jiangxi Provincial Children's Hospital. They were divided into monosomy, chimeric and other karyotype groups according to karyotype, and the levels of AMH, INHB, Estradiol (E2), Luteinising Hormone (LH) and Follicle-Stimulating Hormone (FSH) were analysed according to karyotype. To assess the expression levels of AMH, INHB levels in patients with and without spontaneous puberty and the correlation with spontaneous puberty. To assess ovarian reserve function in TS patients by analysing serum AMH, INHB using Receiver operator characteristic (ROC).

Results:

• 1. The differences in AMH and INHB levels among the three karyotype groups were statistically significant (P <0.05), with a median AMH of 0.09ng/mL and a median INHB of 9.00pg/mL in the monosomy group; 1.87ng/mL in the chimeric group and 15.95pg/mL in the INHB group; and in the other karyotype group, a median AMH of 0.14ng/mL, and median INHB was 11.05pg/mL.

• 2. The differences in AMH and INHB levels were statistically significant in patients with and without spontaneous puberty (P <0.05), and in patients presenting with spontaneous puberty TS, the median AMH was 0.54 ng/mL and the median INHB was 13.70 pg/mL, and the levels of AMH and INHB were positively correlated with spontaneous puberty (P <0.05).

• 3. AMH level was positively correlated with ovarian volume (P <0.05, r =0.444), and INHB level was positively correlated with ovarian volume (P <0.001, r =0.468). The area under the ROC curve of AMH and INHB for predicting the ovarian reserve function was 0.791 (P <0.05). The sensitivity and specificity of AMH was 69.2% and 96.2%, that of INHB was 57.7% and 88.5%.

Conclusions:

• 1. There is a close relationship between serum AMH and INHB levels and karyotype;

• 2. TS patients with spontaneous puberty have higher serum AMH and INHB expression levels than TS patients without spontaneous puberty, and TS patients with high levels of AMH and INHB are more likely to have spontaneous puberty;

• 3. AMH and INHB are important indicators for assessing ovarian reserve function in TS patients;

Key Words: Turner syndrome; karyotype; ovarian reserve function; spontaneous puberty; AMH; INHB