hrp0095p1-186 | Sex Differentiation, Gonads and Gynaecology, and Sex Endocrinology | ESPE2022

Inflammation and polycystic ovary syndrome (PCOS). Utilising proteomic analysis to unravel the link between inflammatory proteins and PCOS in adolescents

Gunn Harriet , Oberg Emma , Hällqvist Jenny , Lindén Hirschberg Angelica , Steinbeck Katharine , Mills Kevin

Background and Methods: PCOS is common in adolescents and imparts a significant health and economic burden. Yet, our understanding of its complex pathogenesis is lacking. Proteomic analyses would be a valuable tool to deepen our knowledge of the biological pathways which underpin PCOS, and to identify novel biomarkers to diagnose and monitor this common condition. Therefore, we undertook a deep phenotyping discovery proteomic profiling (nano-2D-LC-QTOF MSe) on...

hrp0092rfc11.3 | Pituitary, Neuroendocrinology and Puberty Session 2 | ESPE2019

Polycystic Ovarian Syndrome in Adolescents: Utilising Discovery Proteomics and the Search for to Identify Novel Non-Invasive Biomarkers

Gunn Harriet , Forysth Vhari , Hällqvist Jenny , Viner Russell , Mills Kevin , Steinbeck Katharine

Background: Polycystic ovarian syndrome (PCOS) is common, affecting up to one-fifth of females. PCOS is associated with significant comorbidity including metabolic dysfunction, pro-inflammation and mood disorders. Despite this, it is poorly understood, and diagnosis and management remain challenging in adolescents. Proteomics enables the better understanding of disease mechanisms and facilitates the identification of novel biomarkers.<st...

hrp0089p1-p244 | Sex Differentiation, Gonads and Gynaecology or Sex Endocrinology P1 | ESPE2018

Polycystic Ovarian Syndrome in Adolescents: Characterising the Clinical Phenotype and the Role of Precision Medicine

Gunn Harriet , Agarwalla Vathsala , Skinner Rachel , Milne Bronwyn , Mills Kevin , Steinbeck Katharine

Background: Polycystic ovarian syndrome (PCOS) is the most common hormone disorder in adolescent and young adult females, affecting 4–20% of the population. PCOS is associated with metabolic dysfunction, pro-inflammatory processes and mood disorders. Despite this, it is poorly understood in younger adolescents, and diagnosis and management remain challenging.Objectives: To better understand the clinical phenotype of PCOS in adolescents. Subsequently...

hrp0082fclb2 | Late Breaking Abstracts | ESPE2014

Sonic Hedgehog is Required for Cell Specification of Rathke’s Pouch Progenitors During Normal Development and is Over-Expressed in Adamantinomatous Craniopharyngioma

Carreno Gabriela , Andoniadou Cynthia , Heywood Wendy , Mills Kevin , Dattani Mehul , Martinez-Barbera Juan Pedro

Background: Mouse studies have demonstrated the necessity of Sonic Hedgehog (SHH) for normal proliferation of Rathke’s pouch (RP) precursors. However, the possible function of SHH in pituitary cell specification remains to be assessed. Additionally, evidence suggests that SHH may be relevant in human adamantinomatous craniopharyngioma (ACP), a histologically benign, but clinically aggressive childhood tumour associated with high morbidity.Objective ...

hrp0082p2-d2-338 | Diabetes (1) | ESPE2014

Urinary Vitamin E Metabolites as a Biomarker of Oxidative Stress in Type 1 Diabetes

Bulwer Chloe , Hindmarsh Peter , Pope Simon , Tollerfield Sally , Sirka Ernestas , Mills Kevin

Background: Oxidative stress has been implicated in the development and progression of complications in type 1 diabetes (T1DM). Vitamin E (α-tocopherol) undergoes β-oxidation of its chomanol ring and the resulting metabolite α-TLHQ has been proposed as a potential biomarker of oxidative stress. HbA1c relates in T1DM to microvascular complications predominantly although the end-points are late in disease development. The oxidative stress process may act independe...

hrp0094p2-404 | Sex differentiation, gonads and gynaecology or sex endocrinology | ESPE2021

Is inflammation the major driver of polycystic ovary syndrome (PCOS)? A proteomic approach to understanding PCOS in adolescents and the search for novel non-invasive biomarkers

Harriet Gunn/ , Hällqvist Jenny , Doykov Ivan , Steinbeck Katharine , Mills Kevin

Background and Methods: PCOS is common and associated with significant comorbidity. However, its pathogenesis is complex and poorly understood. We have developed new methods for deep phenotyping discovery proteomic profiling of urine, aiming to identify disease mechanisms, drug targets and novel non-invasive biomarkers for PCOS in adolescents. Here, we present the baseline proteomic data from our prospective, longitudinal study (n=40). We undertook proteomic analysis ...