hrp0095p2-150 | GH and IGFs | ESPE2022

Alterations in stem cell populations during rIgF-1 (Increlex) therapy in patients with SPIGF1D

Bossowski Artur , Grubczak Kamil , Stożek Karolina , Starosz Aleksandra , Bossowski Filip , Moniuszko Marcin

Pathway involving insulin-like growth factor 1 (IGF-1) plays significant role in growth and development. Crucial role of IGF-1 was discovered inter alia through studies involving deficient patients with short stature, including SPIGFD individuals. Noteworthy, despite disturbances in proper growth, elevated values for selected stem cell populations were found in IGF-1 deficient patients. Therefore, here we focused on investigating role of these cells - very small embryonic-like...

hrp0095p2-279 | Thyroid | ESPE2022

Evaluating the role of circulating dendritic cells in methimazole-treated pediatric Graves’ disease patients

Bossowski Artur , Grubczak Kamil , Starosz Aleksandra , Stozek Karolina , Bossowski Filip , Moniuszko Marcin

Graves’ disease (GD) is hyperthyroidism associated with organ-specific autoimmune inflammation. GD occurs more frequently in adults than in children, however, pediatric patients are a therapeutic challenge due to cycles of remissions and relapses requiring constant monitoring at every stage of treatment administered. Dendritic cells (DCs) are considered a link between innate and adaptive immunity. DCs as antigen-presenting cells (APCs) are involved in antigen presentatio...

hrp0095p2-281 | Thyroid | ESPE2022

Effects of therapy on Th1, Th17, Th22 and Bregs in pediatric patients with Graves' disease

Stożek Karolina , Grubczak Kamil , Starosz Aleksandra , Bossowski Filip , Moniuszko Marcin , Bossowski Artur

Introduction: The precise pathogenesis of Graves’ disease (GD) still remains unclear, especially in the field of immunological aspects. Thyroid infiltration by reactive T and B lymphocytes plays a crucial role in the course of autoimmune thyroid diseases (ATD). Previous pattern of inflammation process was characterized by the presence of two antagonistic groups of T effector or also called- helper cells: Th1 and Th2. Recently, more attention is paid to T...

hrp0092fc5.2 | Thyroid | ESPE2019

Lower Proportion of CD19+IL-10+ and CD19+CD24hiCD27+ IL-10+, but not CD1d+CD5+CD19+CD24+CD27+ IL-10+ B Cells in Children with Autoimmune Thyroid Diseases

Stozek Karolina , Grubczak Kamil , Marolda Viviana , Eljaszewicz Andrzej , Moniuszko Marcin , Bossowski Artur

Introduction: Hashimoto's thyroiditis (HT) and Graves' disease (GD) become increasingly common in children's population. Pathogenesis of autoimmune thyroid diseases (AITD) bases on coexistence of genetic predisposition and environmental triggers which finally drive to breakdown of immune tolerance. Many mechanisms in human body moderate process of inflammation. While some of them answer for up-regulation, some agents like B regulatory lymphocytes (...

hrp0086fc11.4 | Thyroid | ESPE2016

Decreased Proportions of CD4+IL17+/CD4+CD25+CD127− and CD4+IL17+/CD4+CD25+CD127-FoxP3+T Cells in Children with Autoimmune Thyroid Diseases

Bossowski Artur , Moniuszko Marcin , Grubczak Kamil , Snight Paulina , Bossowska Anna , Diana Tanja , Kahaly George

Background: Until now, altered balance of Th1 and Th2 immune cells has been postulated to play an important role in the pathogenesis of autoimmune thyroid diseases (AITD). However, recent studies on thyroid diseases have suggested a new role for Th17 cells that have been classified as a new lineage, distinct from Th1, Th2 and Treg cells. Despite wide interest, the role of Th17 cells in the pathogenesis of inflammatory and autoimmune diseases is still debated.<p class="abst...

hrp0084p2-566 | Thyroid | ESPE2015

Analysis of B Regulatory Cells with Phenotype CD19+CD24hiCD27+IL-10+ and CD19+IL-10+ in the Peripheral Blood of Children with Graves’ Disease and Hashimoto’s Thyroiditis

Bossowski Artur , Grubczak Kamil , Singh Paulina , Radzikowska Urszula , Dabrowska Milena , Sawicka Beata , Bossowska Anna , Moniuszko Marcin

Background: Autoimmune thyroid disease (AITD) is the most common organ-specific autoimmune disorder. Genetic background, environmental and endogenous factors are play important roles in determining the activation of immune cells or the efficacy of the immunoregulatory pathways. Recently emphasizes the immunosuppressive role of B regulatory cells (phenotype CD19+CD24hiCD27+IL-10+, CD19+IL-10+) in regulation of immune respon...

hrp0097p1-197 | Thyroid | ESPE2023

Effects of methimazole therapy on effector T and B regulatory cells in pediatric patients with Graves' disease

Stożek Karolina , Grubczak Kamil , Starosz Aleksandra , Bossowski Filip , Moniuszko Marcin , Bossowski Artur

Introduction: The incidence of autoimmune thyroid diseases (ATD) is constantly rising. Graves’ disease (GD) remains the most common cause of thyrotoxicosis in pediatric population, but the exact pathogenesis of GD is still not fully understood. The immunological basis of ATD assumes an imbalance between effector and regulatory T lymphocytes. B regulatory cells (Breg) are able to suppress the pro-inflammatory properties of effector T cells. Methimazole (M...

hrp0086p1-p640 | Growth P1 | ESPE2016

Analysis of Correlation between Stem Cells (CD133+/CD45+ and CD133+/CD45) and Anthropometric Parameters of Children with Growth Hormone/Primary Insulin-Like Growth Factor 1 Deficiency

Sawicka Beata , Moniuszko Marcin , Grubczak Kamil , Singh Paulina , Radzikowska Urszula , Mikłasz Paula , Dębrowska Milena , Bossowski Artur

Introduction: Hematopoetic progenitor stem cells (HSCs, CD133+/CD45+) and very small embryonic-like stem cells (VSELs, CD133+/CD45−) can differentiate into specific immune cells. Some studies suggest that levels of HSCs and VSELs change during therapy with growth hormone (GH) or insulin-like growth factor 1 (IGF-1). GH deficiency (GHD), an endocrine disease connected with insufficient production of GH by pituitary gland, is tre...

hrp0089p1-p149 | GH &amp; IGFs P1 | ESPE2018

Assesment of SDF-1 and Ang-1 and Ang-2 in Children with Growth Hormone Deficiency before and after 1- year Therapy with Recombinant Growth Hormone

Sawicka Beata , Moniuszko Marcin , Grubczak Kamil , Singh Paulina , Radzikowska Urszula , Mikłasz Paula , Dębrowska Milena , Borysewicz-Sańczyk Hanna , Bossowski Artur

Introduction: Angiopoietins are necessary for development, differentiation and stabilization vessels progress. Angiopoient 1 (Ang-1) is responsible for vascular integrity, through stimulation of endothelial cell migration and adhesion, and inhibition of apoptosis. Action of angiopoietin 2 (Ang-2), in the absence of VEGF it Leeds to vascular regression, but in the presence of high VEGF concentration it stimulates angiogenesis. Stromal derived factor (SDF-1) play an important ro...