ESPE Abstracts (2014) 82 P-D-3-1-766

Positive Association of Pro-Oxidative Stress Markers with Adipose Mass in Pre- and Early-Pubertal Boys

George Paltogloua,b, Maria Schoinac, George Valsamakisa, Alexandra Avlonitic, Athanasios Chatzinikolaouc, Antonis Kambasc, Dimitris Draganidisc, Ioannis Papassotirioud, Alexandra Margelid, Nicolaos Salakose, Maria Papagiannif, Christina Kanaka-Gantenbeinb, George P Chrousosb, Ioannis G Fatourosc & George Mastorakosa

aEndocrine Unit, Second Department of Obstetrics and Gynecology, Aretaieion Hospital, Athens University Medical School, Athens, Greece; bFirst Department of Pediatrics, ‘Aghia Sofia’ Children’s Hospital, Athens University Medical School, Athens, Greece; cSchool of Physical Education and Sports Sciences, Democritus University of Thrace, Komotini, Greece; dDepartment of Biochemistry, ‘Aghia Sofia’ Children’s Hospital, Athens, Greece; eSecond Department of Obstetrics and Gynecology, Aretaieion Hospital, Athens University Medical School, Athens, Greece; fPediatric Endocrinology Unit, Third Department of Pediatrics, Hippokrateion General Hospital of Thessaloniki, Aristotle University of Thessaloniki, Thessaloniki, Greece

Background: Oxidative stress and adipocytokines are associated with obesity.

Objective and hypotheses: To investigate the association of adipocytokines with markers of pro- and anti-oxidation at baseline and their change and correlations during acute aerobic exercise in normal weight and obese pre- and early-pubertal boys.

Method: Fifty healthy pre- and early-pubertal, normal weight and obese boys, underwent a baseline blood sampling followed by an aerobic exercise bout until exhaustion at 70% VO2max and a subsequent (post-exercise) sampling for the measurement of pro-oxidation markers (TBARS, PCs); anti-oxidation markers (GSH, GSSG, GPX, catalase, and TAC) and adipocytokines (lipocalin-2, RBP4, hsCRP, hsIL6, adiponectin, and leptin).

Results: No difference was found between pre- and early-pubertal subjects in pro- and anti-(except baseline TAC) oxidation markers and adipocytokine concentrations. Baseline pro-oxidation markers, hsCRP, hsIL6, and leptin concentrations were greater in obese than normal weight subjects, whereas the reverse was true for anti-oxidation markers. Post-exercise concentrations of pro- and anti-oxidation markers, RBP4 and hsIL6 were significantly different compared to baseline in all subjects groups. In all subjects baseline TBARS and PCs correlated positively with leptin and hsCRP while post-exercise PCs correlated positively with post-exercise hsIL6. Also, baseline TAC correlated negatively with RBP4 and hsIL6 while baseline GSH correlated negatively with hsIL6, hsCRP, and leptin. Furthermore, baseline and post-exercise GPX was negatively correlated to hsCRP and hsIL-6 respectively.

Conclusion: Pro-oxidation markers, inflammatory adipocytokines and leptin are greater in obese compared to normal-weight pre- and early-pubertal boys whereas anti-oxidation markers are greater in normal weight boys. Post-exercise both pro- and anti-oxidative stress markers change significantly in all subjects. In all subjects pro-and anti-oxidation markers are positively and negatively correlated, respectively, with inflammatory adipocytokines and leptin, a marker of adipose mass. These findings indicate the deleterious association of pro-oxidation with adipose tissue in pre- and early-pubertal boys.