ESPE Abstracts (2015) 84 P-3-630

CTLA4 A49G and C60T Genetic Polymorphism in Croatian Children and Young Adults with Autoimmune Thyroid Disease

Natasa Rojnic Putareka, Vesna Kusecb, Zorana Grubicc, Jadranka Knezevic-Cucad, Jasenka Illea, Biserka Stajnklera, Bruna Jaksice & Miroslav Dumica


aDepartment of Pediatric Endocrinology and Diabetes, University Hospital Center Zagreb, Zagreb, Croatia; bDepartment of Laboratory Diagnostics, University Hospital Center Zagreb, Zagreb, Croatia; cTissue Typing Centre, University Hospital Center Zagreb, Zagreb, Croatia; dInstitute of Clinical Chemistry and Laboratory Medicine, Merkur University Hospital Zagreb, Zagreb, Croatia; eFaculty of Electrical Engineering and Computing, University of Zagreb, Zagreb, Croatia


Background: Autoimmune thyroid disease (AITD), including autoimmune thyroiditis (AT) and Graves’ disease (GD), is a complex autoimmune disease with a strong genetic component. The cytotoxic product of T-lymphocyte antigen-4 (CTLA4) gene, encoding a negative regulator of the T-lymphocyte immune response, was shown to be associated to AITD.

Objective and hypotheses: To investigate the association of A49G and C60T polymorphisms of CTLA4 gene in population of Croatian children and young adults with AITD.

Method: The study comprised of 158 unrelated AITD patients (36 males, 122 females) with median age of 12.5 years (4.2–25.9), 127 with AT and 31 with GB. The control group consisted of 94 unrelated healthy subjects (46 males, 48 females) with median age of 12.0 years (4.6–21.5). SNP genotyping was performed using TaqMan probes (rs231775 and rs3087243) in a PCR ABI PRISM 7500 Sequence Detection System (Applied Biosystems by LT).

Results: A49G disease associated G/G genotype of CTLA4 gene was detected more frequently in AITD patients (19.6%; OR 1.67, 95% CI 0.81–3.43; P=0.16), in AT patients (20.5%, OR 1.76, 95% CI 0.84–3.70, P=0.13) and GD patients (16.1%, OR 1.31, 95% CI 0.42–4.08, P=0.76) as compared to controls (12.8%), but no statistical significance for associations in none of the groups was found. Significant associations were found for the C60T disease-associated G/G genotype and AITD (OR 2.23, 95% CI 1.26–3.95; P<0.01), mainly for AT (OR 2.42, 95% CI 1.34–4.38; P=0.003), but not GB (OR 1.56, 95% CI 0.64–3.80; P=0.33). The risk allele G of both polymorphisms (A49G and C60T) was not significantly associated to AITD (P=0.47 and P=0.10 respectively).

Conclusion: Our results do not indicate association of CTLA4 gene G49A polymorphism in the pathogenesis of AITD. However, the C60T polymorphism of CTLA4 gene was found to be associated to the risk of AITD, particularly to AT in our population.

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