ESPE Abstracts (2015) 84 P-3-873

Sex Differences in the Pubertal Response to High-Fat Diet

Alejandra Freire-Regatilloa,b, Pilar Argente-ARizóna,b, Francisca Diaza, Vicente Barriosa, Angeles Arevaloc, Jesús Argentea,b, Luis Miguel García-Segurac, Iñigo Azcoitiad & Julie A Chowena

aDepartment of Endocrinology, Instituto de Investigación la Princesa, CIBEROBN, Hospital Infantil Universitario Niño Jesús, Madrid, Spain; bDepartment of Paediatrics, Universidad Autónoma de Madrid, Madrid, Spain; cInstituto Cajal, CSIC, Madrid, Spain; dDepartment of Biological Science, Universidad Complutense, Madrid, Spain

Background: It is well known that ingestion of a high fat diet (HFD) can induce rapid weight gain and metabolic imbalances. However, males and females are not equally susceptible to these effects. Furthermore, an individual may be more prone to gain weight during specific developmental periods.

Aims and objectives: We aimed to analyse the response to the acute exposure to a HFD during pubertal/adolescent period and to determine whether males and females respond differently.

Methods: Adolescent C57bl/6 mice of both sexes were placed on a HFD or low fat diet (LFD) for eight days. Body weight, adipose tissue mass and glycaemia were measured at sacrifice and serum was collected. Serum insulin, leptin, interleukin (IL)6 and TNFα levels were determined by multiplex assays.

Results: A greater number of kilocalories were ingested by the HFD group compared to the LFD groups of both sexes, with this being significant in females (P<0.03), and with males eating more than females regardless of diet (P<0.0001). No significant effect on weight gain or adipose tissue mass was found. However, HFD increased glycaemia in males (HFD: 132±7 vs LFD: 100±5 mg/dl; P<0.002), but not in females (HFD: 106±6 vs 100±3 mg/dl). Although insulin, leptin and TNFα levels increased with the HFD, especially in males, this was not significant. In contrast, IL6 levels decreased with HFD in males (P<0.002).

Conclusion: During the pubertal/adolescent period in mice, there is a rapid response to HFD intake with males being more susceptible than females.

Funding: This work was funded by grants from Fondos de Investigación Sanitaria (PI100747; PI1302195), Ministerio de Ciencia e Innovación (BFU2011–27492), Centro de Investigación Biomédica en Red Fisiopatología de Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, and Fundación de Endocrinología y Nutrición.

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