ESPE Abstracts (2018) 89 P-P2-159

Serum NAMPT Levels are not Associated with Parameters of Liver Function in Children and Adolescents

Melanie Penkea, Susanne Schusterb, Yvonne Dietzc, Antje Gartena,d, Nico Grafec, Thomas Karlase, Johannes Wiegande, Antje Körnera,c,f & Wieland Kiessa,c,f

aCenter for Pediatric Research Leipzig, Leipzig, Germany; bDepartment of Cardiology, Leipzig University Hospital, Leipzig, Germany; cLIFE-Child- Leipzig Research Center for Civilization Diseases, Leipzig, Germany; dInstitute of Metabolism and Systems Research, Birmingham, UK; eDepartment of Gastroenterology and Rheumatology, Leipzig University Hospital, Leipzig, Germany; fUniversity Hospital for Children and Adolescents, Leipzig, Germany

Background/Aim: Serum NAMPT (nicotinamide phosphoribosyltransferase) levels are altered in adult patients with non-alcoholic fatty liver disease (NAFLD). However, less is known about NAMPT serum levels children and adolescents and their association with parameters of liver function.

Methods: Blood and anthropometric data of 416 children and adolescents who participated in the LIFE Child Study Leipzig were collected. Serum NAMPT (Adipogen) and cytokeratin-18 (PEVIVA®, TECOmedical) levels were measured by ELISA Kit. Vibration-Controlled Transient Elastography (VCTE) and Controlled Attenuation Parameter (CAP) (FibroScan®, M Probe, 10 successful measurements) were used to define the liver fibrosis and steatosis, respectively. Association between NAMPT serum levels with liver parameters (alanine aminotransferase (ALAT), γ-glutamyltransferase (GGT), aspartate aminotransferase (ASAT), VCTE, CAP, cytokeratin-18 (CK18), pediatric NAFLD score) as well as metabolic and inflammatory markers were calculated by Spearman’s correlation or multiple regression analysis. Differences between groups were calculated by Mann–Whitney U test.

Results: We found no differences in serum NAMPT levels between girls and boys. A positive correlation of NAMPT with anthropometric data such as BMI [kg/m2] (r=0.141, P=0.005) and hip circumference [cm] (r=0.132, P=0.01) was detected in all children and adolescents. Alanine aminotransferase level (ALAT) [U/l] were positively correlated with serum NAMPT, especially in children with obesity (r=0.211, P=0.02). In this subgroup, γ-glutamyltransferase (GGT) [U/l] (r=0.333, P=0.021) and CK18 (r=0.205, P=0.015) showed a positive correlation with NAMPT in serum However, no correlation could be found with liver steatosis and fibrosis as measured by FibroScan® as well as the calculated pediatric NAFLD score. Further, circulating NAMPT was correlated with inflammatory markers such as C-reactive protein [mg/l] (r=0.252, P=0.000), leucocyte count [109/l] (r=0.350, P=0.000) and neutrophil count [109/l] (r=0.298, P=0.000), in particular in children with overweight (C-reactive protein: r=0.361, P=0.014; leucocyte count: r=0.467, P=0.007; neutrophil count: r=0.375, P=0.007) and obesity (C-reactive protein: r=0.326, P=0.000, leucocyte count: r=0.336, P=0.000). After multiple regression analysis and adjustment on BMI-SDS, age and sex, association between NAMPT and inflammatory markers but not liver parameters remained, especially leucocyte count, in all children and adolescents (R2=0.126, P=0.000) as well as in children and adolescents with overweight (R2 =0.289, P=0.002).

Discussion: Our data show that serum NAMPT levels in children and adolescents are not associated parameters of liver dysfunction. We could confirm previous studies that showed a positive association with NAMPT serum levels and inflammatory markers in children and adolescents.

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