ESPE Abstracts (2019) 92 P3-89

A Mitophagic Response to iron Overload-Induced Oxidative Damage Associated with the PINK1/Parkin Pathway in Pancreatic Beta Cells

Lina ZHANG, Liyang LIANG, Zulin LIU, Lele HOU, Hui OU, Siqi HUANG

Sun Yat-sen Memorial Hospital, Sun Yat-sen University, GUANGZHOU, China

An increased iron overload led to a disorder in glucose metabolism. However, the mechanism through which iron overload induces beta cell death remains unknown. The present study revealed that ferric ammonium citrate treatment inhibited cell viability in vitro, induced a decline in mitochondrial membrane potential, increased oxidative stress and activated mitophagy. These effects could be alleviated by a reactive oxygen species scavenger. In summary, we demonstrated that increased iron overload induced cytotoxicity in INS-1 cells primarily by activating oxidative stress and further triggering mitophagy in the PTEN-induced putative kinase 1/Parkin pathway. These findings may shed some light on the mechanism of iron toxicity-mediated pancreatic beta-cell dysfunction.

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