An increased iron overload led to a disorder in glucose metabolism. However, the mechanism through which iron overload induces beta cell death remains unknown. The present study revealed that ferric ammonium citrate treatment inhibited cell viability in vitro, induced a decline in mitochondrial membrane potential, increased oxidative stress and activated mitophagy. These effects could be alleviated by a reactive oxygen species scavenger. In summary, we demonstrated that increased iron overload induced cytotoxicity in INS-1 cells primarily by activating oxidative stress and further triggering mitophagy in the PTEN-induced putative kinase 1/Parkin pathway. These findings may shed some light on the mechanism of iron toxicity-mediated pancreatic beta-cell dysfunction.
19 - 21 Sep 2019
European Society for Paediatric Endocrinology