ESPE Abstracts

Objectives: To compare the levels of E2 in girls measured by LC-MS/MS and CLIA, and to evaluate the correlations between E2 levels and bone age, uterine length, and uterine volume.

Methods: 133 newly diagnosed girls were selected. There were 80 girls in the ICPP group (44 in the Tanner II stage in the ICPP group, 36 in the Tanner III in the ICPP group), and 53 in the PT group， 40 healthy girls in the Tanner III group, bone age, and uterus and ovary ultrasound results were collected, and CLIA and LC-MS/MS methods detected essential serum E2.

Results: Among the 173 girls, 11 cases (6.4%) of E2 measured by CLIA were less than the lower limit of detection (43.31pmol/l), and two patients (1.2%) of E2 measured by LC-MS/MS were less than the lower limit of detection (3.70pmol/l). Among the PT group, 5 cases (9.4%) of E2 measured by CLIA were less than the lower limit of detection, and 2 patients (3.8%) of E2 measured by LC-MS/MS were less than the lower limit of detection. Among the Tanner II stage of the ICPP group, E2 measured by CLIA was less than the lower detection limit in 3 cases (6.8%), and E2 by LC-MS/MS was not less than the lower limit of detection. Among the Tanner III stage of the ICPP group, 1 case (2.8%) of E2 measured by CLIA was less than the lower detection limit, and none of E2 by LC-MS/MS was less than the lower limit of detection. In the normal puberty Tanner III group, 2 cases (5%) of E2 measured by CLIA were less than the lower limit of detection, and by LC-MS/MS was not less than the lower limit of detection. In the PT、Tanner II of the ICPP、the Tanner III of the ICPP and the Tanner III of normal puberty group, the basal E2 level measured by CLIA was significantly higher than that measured by LC-MS/MS in the same group. From a clinical point of view, the difference between the results of the two detection methods is too significant. It can not be accepted in a clinic.

Conclusions: The E2 level detected by LC-MS/MS method differs from that seen by the CLIA method. LC-MS/MS method has higher sensitivity and lower E2 class, and LC-MS/MS method can quantitatively detect E2 in a lower concentration range.