ESPE Abstracts

Objective: The onset of obesity complicated with metabolic syndrome in children and adolescents is hidden, the mechanism is unknown, and early warning indicators are lacking clinically. This study conducted multi-omics research on children and adolescents with simple obesity and obesity complicated with metabolic syndrome to provide early clinical diagnosis and drug treatment targets for its occurrence and development mechanism.

Methods: We performed proteomics, metabolomics, protein phosphorylation, N-glycosylation and other multi-omics methods are used for detection, and statistical analysis such as differential screening is used.

Results: 1) Serum metabolomics results suggested that MetS (n=40) and Con (n=40) HMDB classification analysis suggested that lyso-paf c-18, 1-heptadecanoyl-sn-glycero-3-phosphocholine, palmitic amide The most obvious down-regulation is 5alpha-androst-16-en-3-one, indoline, 3-dehydroepiandrosterone sulfate and the most obvious up-regulation; OB (n=40) and Con (n=40) HMDB classification analysis suggests that 1-heptadecanoyl-sn-glycero -3-phosphocholine, lyso-paf c-18, and palmitic amide were most significantly down-regulated, and decanoyl-l-carnitine, dodecanoylcarnitine, and eicosapentaenoic acid were most significantly up-regulated. 2) Serum proteomics results suggest that, compared with MetS (n=20) and Con (n=20) differential proteomics, the down-regulation of SHBP, PON3, LSAMP is the most obvious, and the up-regulation of FAH, PRG4, APOL1 is the most obvious; OB (n= 20) Compared with Con (n=20) differential proteomics, the down-regulation of SCG3, SHBG and IGFBP2 was the most obvious, and the up-regulation of COL18A1, FAH and CFD was the most obvious. 3) The omics results of serum 4D Label free protein N glycosylation showed that differential screening of MetS (n=20) and Con (n=20) indicated that A1BG, HYOU1, and C1QA were most significantly up-regulated, and APOC4, SPARCL1, and LAMP1 were most down-regulated obvious. Differential screening between OB (n=20) and Con (n=20) revealed that ORM2, ORM1, and ALPL were most significantly up-regulated, and CFH, LAMP2, and COL1A1 were most significantly down-regulated. 4) The results of serum 4D Label free protein phosphorylation modified omics research showed that GOLM1, ALPL, and TF were most significantly up-regulated, and APOA1, C4BPA, and ITIH4 were most significantly down-regulated.

Conclusion: The results of this study suggest that compared with simple obesity, adolescents with obesity complicated with metabolic syndrome have different differential proteins, which may be used as drug targets for subsequent treatment.