hrp0092fc7.5 | Diabetes and Insulin Session 2 | ESPE2019

Is the 1-hour Post-Load Glucose Level by 75g Oral Glucose Tolerance Test a New Risk Factor in Predicting Atherosclerosis?

Kilinç Suna , Demirtaş Tuna , Atay Zeynep

Background and Objectives: Recent studies demonstrated that a 1-hour post-load plasma glucose (1-h PG) value of ≥155 mg/dL during an oral glucose tolerance test (OGTT) is a new risk factor for predicting atherosclerosis. Herein, we evaluated whether individuals with normal glucose tolerance (NGT), whose 1-h PG is ≥155mg/dl, or with impaired glucose tolerance (IGT) have an increased carotid intima–media thickness (CIMT), as compared with NGT in...

hrp0092fc7.6 | Diabetes and Insulin Session 2 | ESPE2019

2017 American Academy of Pediatrics Clinical Practice Guideline: Impact on Prevalence of Arterial Hypertension in Children and Adolescents with Type 1 Diabetes mellitus

Dost Axel , Bechtold Susanne , Fink Katharina , Bonfig Walter , Wiemann Dagobert , Kapellen Thomas Michael , Witsch Michael , Schwab Karl Otfried , Holl Reinhard Walter

Background: In 2017 the American Academy of Pediatrics has introduced a new guideline (AAP 2017) to diagnose arterial hypertension in children, as the blood pressure thresholds for adults had been lowered before. There is a controversy about these new reference levels as other societies have not followed these recommendations. We studied the impact of the new AAP 2017 guideline on prevalence of arterial hypertension (HTN) in children with Type 1 diabetes melli...

hrp0092fc8.1 | Pituitary, Neuroendocrinology and Puberty Session 1 | ESPE2019

Hypothalamic AgRP Neurons Drive Endurance in Food-restricted Mice

Miletta Maria Consolata , Horvath Tamas L.

Diseases of food restriction, such as anorexia and bulimia nervosa, are psychiatric conditions with the highest mortality. It is not known how these disorders emerge and what determine mortality. Individuals with these disorders frequently engage in compulsive exercise. States of food restriction are associated with elevated activity of hypothalamic neurons that produce AgRP, which cells are crucial for feeding and can promote stereotypic behaviors.Here,...

hrp0092fc8.2 | Pituitary, Neuroendocrinology and Puberty Session 1 | ESPE2019

Analysis of Hypothalamic Metabolic Circuits after Normalization of Body Weight in Mice That Had Been Obese Due to High Fat Diet Intake

Guerra-Cantera Santiago , Frago Laura , Ros Purificación , Canelles Sandra , Díaz Francisca , Freire-Regatillo Alejandra , Torrecilla-Parra Marta Marta , Argente Jesús , Chowen Julie A.

The obesity epidemic continues to be a dramatic problem in the developed world despite attempts to curtail its rise. Reducing energy intake and/or increasing energy expenditure can result in weight loss; however, if one returns to their poor lifestyle habits the previous weight is not only recuperated, but often surpassed.We hypothesized that although a normal body weight may be achieved, the hypothalamic circuits controlling appetite and energy expendit...

hrp0092fc8.3 | Pituitary, Neuroendocrinology and Puberty Session 1 | ESPE2019

Absence of Central Adrenal Insufficiency in Adults with Prader-Willi Syndrome

Rosenberg Anna , Davidse Kirsten , Pellikaan Karlijn , Donze Stephany , Hokken-Koelega Anita , van der Lely Aart Jan , de Graaff Laura

Introduction: Individuals with Prader-Willi syndrome (PWS) suffer from hyperphagia, hypotonia and hypothalamic dysfunction, leading to a variety of pituitary hormone deficiencies. Central adrenal insufficiency (CAI) has been reported in PWS, while each of these studies used different testing modalities and cut-off values. Therefore, reported prevalence of CAI ranges from 0% to 60%. It has been speculated that CAI might be responsible, at least in part,...

hrp0092fc8.4 | Pituitary, Neuroendocrinology and Puberty Session 1 | ESPE2019

Peripheral and Hypothalamic Alterations in the Insulin-like Growth Factor (IGF) System in Response to High Fat Diet-induced Weight Gain

Guerra-Cantera Santiago , Díaz Francisca , Ros Purificación , Freire-Regatillo Alejandra , Torrecilla-Parra Marta , Barrios Vicente , Frago Laura , Chowen Julie A. , Argente Jesús

The insulin-like growth factor (IGF) system is fundamental for physiological processes such as growth and metabolism. In addition, in the brain it regulates glucose metabolism and neuroprotection. The IGF axis can be altered by nutritional status, but little is known regarding the effects of specific dietary components on this system.Our aim was to examine how high-fat diet (HFD) and low-fat/high sucrose diet (LFHSD) intake affect the central and circula...

hrp0092fc8.5 | Pituitary, Neuroendocrinology and Puberty Session 1 | ESPE2019

LGR4-Wnt β-Catenin Signalling Directs GnRH Network Development, with Defects Leading to Self-Limited Delayed Puberty

Mancini Alessandra , Howard Sasha R. , Cabrera Claudia P. , Barnes Michael R. , David Alessia , Wehkalampi Karoliina , Vassart Gilbert , Cariboni Anna , Garcia Marie Isabelle , Guasti Leonardo , Dunkel Leo

Background: The initiation of puberty is dependent upon an augmentation of gonadotropin-releasing hormone (GnRH) secretion from the hypothalamus. Development of the GnRH neuroendocrine network in embryonic life depends on coordinated migration of neurons from the vomeronasal organ in the nose to the forebrain. We have previously demonstrated that dysregulation in GnRH neuronal migration leads to delayed pubertal onset. Late puberty affects up to 2% of the ...

hrp0092fc8.6 | Pituitary, Neuroendocrinology and Puberty Session 1 | ESPE2019

Source and Changes in Serum Level of Kisspeptin in Female Rats at Different Developmental Stages

Kwon Ahreum , Kim Ho-seong , Suh Jungwhan , Choi Han-saem , Song Kyungchul , Suh Yongsuk

Introduction: The Kiss1/Kisspeptin/Kiss1r system is essential for puberty onset and reproductive system development, especially in the hypothalamus. Nevertheless, Kiss1 is expressed in other organs. Additionally, serum kisspeptin has been associated with puberty. However, studies on the developmental changes in serum kisspeptin levels and its main source are limited. Therefore, the aim of this study was to evaluate the developmental ...

hrp0092fc9.1 | Fetal, Neonatal Endocrinology and Metabolism (to include Hypoglycaemia) | ESPE2019

Using CRISPR/Cas9 Gene Editing to Study the Molecular Mechanisms of Congenital Hyperinsulinism (CHI)

Purushothaman Preetha , Walker Amy , Maeshima Ruhina , Hussain Khalid , Hart Stephen

Background: Congenital Hyperinsulinism(CHI) is characterized by the unregulated secretion of insulin in the presence of hypoglycaemia. The mutations in ABCC8 and KCNJ11, which encode the sulfonylurea receptor 1 (SUR1) and potassium inward-rectifying 6.2 (Kir6.2) subunits of ATP-sensitive potassium channel (K channel), are the most common identified cause of the condition. Defects in the HADH gene are responsible for SCHAD- HI, a rare form of the disease caused...

hrp0092fc9.2 | Fetal, Neonatal Endocrinology and Metabolism (to include Hypoglycaemia) | ESPE2019

Heterozygous Insulin Receptor (INSR) Mutation associated with Neonatal Hyperinsulinaemic Hypoglycaemia and Familial Diabetes Mellitus

Sethi Aashish , Ahmed Syed Haris , Colclough Kevin , Didi Mohammed , Flanagan Sarah , Senniappan Senthil

Introduction: Mutations in Insulin Receptor (INSR) is usually associated with insulin resistance and hyperglycemia. Homozygous or compound heterozygous mutations in INSR are usually linked with Rabson-Mendenhall or Donohue syndromes whilst heterozygous INSR mutations are associated with type A insulin resistance. Various autosomal dominant heterozygous INSR mutations leading to hyperinsulinemic hypoglycemia (HH) have been de...