ESPE Abstracts

Myokines - biologically active proteins produces and secretes by skeletal muscles in response to physical activity (PA). They play a role in lipid and glucose metabolism, myogenesis and osteogenesis, thermogenesis activity. Exercise-induced expression of myokines in adolescents with simple obesity insufficiently studied.

Objective: to determine which intensity and duration of PA maximally changes the level of myokines (interleukin-6 (IL-6), myostatin, decorin, irisin).

Materials and methods: 26 children (10 male/16 female), age 15 [13;16] years, SDS BMI: +2.91 [2.24;3.29] were divided into two groups: I (n = 13) was performed PA (walking on a treadmill under heart rate control) duration 30 and 60 minutes at the same intensity (less than 3 metabolic equivalents (MET)) and II (n = 13) was performed PA of different intensity: low (LPA) ― less than 3 MET and moderate (MPA) ― 3-6 MET at the same duration of 45 minutes. Myokines in blood were measured before and after PA.

Results: The groups were matched by age, sex, Tanner stages and basal levels of myokines. IL-6 concentrations were 0.205 [0.141; 0.236] and 0.256 [0.162; 0.344] pg/ml, myostatin 22.19 [18.65; 23.06] and 23.94 [21.53; 25.85] ng/ml, decorin 3.89 [3.036; 4.469] and 4.332 [4.043; 4.743] ng/ml, irisin 33.32 [22.516; 36.348] and 31.896 [22.27; 34.8] mkg/ml. After 30 minutes of PA IL-6 level was increased by 68.3% (P < 0.05). PA for a 60 minutes produce lifting the levels of IL-6, myostatin by 68.3%, 28.8%, but irisin was decreased by 26.7% (P < 0.05 for all). After LPA the levels of IL-6, myostatin, decorin were increased by 132%, 16.7%, 17.1%, irisin was decreased by 16% (P < 0.05 for all). MPA produce elevation the level of IL-6, myostatin, decorin by 151.6%, 30.9%, 34.3%, then irisin was reduced by 21.3% (P < 0.05 for all).

Conclusion: The results demonstrate the effect of PA on myokine secretion in adolescents with obesity. MPA induced an increase in the levels of IL-6, myostatin, decorin, while PA within 60 minutes led to a significant decrease serum levels of irisin.