hrp0094p2-125 | Diabetes and insulin | ESPE2021

Focal Congenital Hyperinsulinism in Infancy is Directly Linked to Increased Numbers of Islet Pancreatic Polypeptide Cells in Islets.

Banerjee Indraneel , Worth Chris , Salomon-Estebanez Maria , Yau Daphne , Jabbar Shamila , Hall Caroline , Dunne Mark ,

Congenital Hyperinsulinism (CHI) is primarily associated with defects in the regulated release of insulin from ß-cells but little information is available about the role of other islet cell types. Pancreatic polypeptide (PP) cells represent a minor component of the islet endocrine cell population. PP causes satiety, decreases gastrointestinal tract motility and suppresses glucagon release. Since CHI is associated with feeding problems and loss of glucagon-mediated counter...

hrp0086rfc10.6 | Perinatal Endocrinology | ESPE2016

Increased Islet Cell Neogenesis and Endocrine Cell Differentiation in Congenital Hyperinsulinism in Infancy

Hardwick Elise , Han Bing , Salomon-Estebanez Maria , Padidela Raja , Skae Mars , Craigie Ross , Cosgrove Karen , Banerjee Indi , Dunne Mark

Background: Congenital Hyperinsulinism in Infancy (CHI) is characterised by inappropriate insulin release. We currently attribute hypoglycaemia to β-cell dysfunction because of defects in the ion channel genes ABCC8 or KCNJ11. However, the CHI pancreas is also associated with inappropriate expression of foetal-like transcription factors and enhanced cell proliferation.Hypothesis: As the CHI pancreas bears similarities to the foetal pancreas, we hypo...

hrp0086p1-p551 | Perinatal Endocrinology P1 | ESPE2016

Enhanced Mitochondrial Densities Associate with the Pathobiology of β-Cells in Congenital Hyperinsulinism in Infancy

Han Bing , Salomon-Estebanez Maria , Padidela Raja , Skae Mars , Kadler Karl , Cosgrove Karen , Banerjee Indi , Dunne Mark

Background: Congenital hyperinsulinism in infancy (CHI) is associated with inappropriate insulin release from β-cells. This is causally linked to defects in the ion channel genes ABCC8 and KCNJ11 regulating insulin, but little is known about the metabolic support for sustained insulin exocytosis.Objective and hypotheses: We hypothesised that inappropriate insulin release in CHI would require sustained ATP generation by enhanced mit...

hrp0086p1-p555 | Perinatal Endocrinology P1 | ESPE2016

Islet of Langerhans in Congenital Hyperinsulinism in Infancy are Disrupted and with Decreased Expression of Collagen (IV) α1 Chain in Basement Membranes

Mal Walaa , Salomon-Estebanez Maria , Padidela Raja , Skae Mars , Craigie Ross , Rigby Lindsey , Cosgrove Karen , Banerjee Indi , Dunne Mark

Background: Congenital hyperinsulinism of infancy (CHI) is the most common cause of severe hypoglycaemia in children. Although CHI arises from mutations in KATP channels which lead to inappropriate insulin secretion, CHI it also is associated with marked changes in islet organization.Aims and objectives: Our aim was to investigate the structure and composition of the islet capsule in CHI and age-matched control tissue.Me...

hrp0084fc9.1 | Beta cell disorders | ESPE2015

Islet δ-Cells Contribute to the Pathobiology of Atypical Congenital Hyperinsulinism

Han Bing , Bourke Siobahn , Mohammad Zainab , Craigie Ross , Skae Mars , Cheeseman Edmund , Banerjee Indi , Cosgrove Karen , Dunne Mark

Background: Atypical forms of congenital hyperinsulinism in infancy (CHI-A) represent a novel subgroup of patients who present later in the neonatal period; have poor responses to medical intervention; an unremarkable histopathology and no known genetic cause of disease.Objective and hypotheses: To compare the expression profiles of insulin and somatostatin in islets from patients with CHI-A, diffuse CHI (CHI-D) and age-matched control tissue.<p clas...

hrp0084fc9.2 | Beta cell disorders | ESPE2015

A Novel Source of Mesenchymal Stem Cells Lines from the Human Neonatal Pancreas of Patients with Congenital Hyperinsulinism in Infancy

Kellaway Sophie , Mosinska Karolina , Han Bing , Mohammad Zainab , Rigby Lindsey , Skae Mars , Padidela Raja , Banerjee Indi , Cosgrove Karen , Dunne Mark

Background: Congenital hyperinsulinism in infancy (CHI) is a neonatal disorder of uncontrolled insulin release leading to profound hypoglycaemia. In addition to defects in pancreatic β-cell function, we have recently demonstrated that the CHI pancreas is highly proliferative, with rates of proliferation up to 14-fold higher than in age-matched controls.Objective and hypotheses: As patients require pancreatectomy to alleviate hypoglycaemia, our aim w...

hrp0084fc9.3 | Beta cell disorders | ESPE2015

Failure to Terminate Cell Proliferation Contributes to the Pathobiology of Congenital Hyperinsulinism in Infancy

Han Bing , Mohammad Zainab , Rigby Lindsey , Craigie Ross , Skae Mars , Padidela Raja , Cheesman Edmund , Cosgrove Karen , Banerjee Indi , Dunne Mark

Background: Diffuse congenital hyperinsulinism in infancy (CHI-D) mainly arises from mutations in KATP channel genes. In addition, there are also several reports of increased cell proliferation in CHI-D. We hypothesised that the higher rates of proliferation in CHI-D are as a consequence of failure to terminate proliferation in the neonatal period.Objective and hypotheses: To test this we examined the proliferative index (PI) of CHI-D tissue a...

hrp0094p1-24 | Diabetes A | ESPE2021

Resolution of feeding problems in patients with congenital hyperinsulinism

Worth Chris , Hall Caroline , Wilson Sarah , Gilligan Niamh , O’Shea Elaine , Salomon-Estebanez Maria , Dunne Mark , Banerjee Indraneel ,

Background: Congenital Hyperinsulinism (CHI) is the most common cause of recurrent and severe hypoglycaemia in childhood and can be broadly categorised into two subtypes. Diffuse CHI (CHI-D) involving all pancreatic cells is usually treated with medications and rarely subtotal pancreatectomy. Focal CHI (CHI-F) involves a solitary insulin hypersecreting pancreatic lesion and can be cured following surgical lesionectomy. Many patients with CHI-F and CHI-D underg...

hrp0094p1-61 | Diabetes B | ESPE2021

Timing of Hypoglycaemia in Patients with Hyperinsulinism (HI): Extension of the Digital Phenotype

Worth Chris , Harper Simon , Salomon-Estebanez Maria , O’Shea Elaine , Nutter Paul , Dunne Mark J , Banerjee Indraneel ,

Background: Hyperinsulinism (HI) due to excess and dysregulated insulin secretion is the most common cause of severe and recurrent hypoglycaemia in childhood. High cerebral glucose utilisation in the early hours results in high risk of hypoglycaemia for people with diabetes and carries a significant risk of brain injury. Prevention of hypoglycaemia is the cornerstone of management for HI but the risk of hypoglycaemia at night or indeed the timing of hypoglycae...

hrp0095rfc3.2 | Early Life and Multisystem Endocrinology | ESPE2022

The Hypoglycaemia Error Grid: a UK-wide Consensus on CGM Accuracy Assessment in Hypoglycaemia due to Congenital Hyperinsulinism

Worth Chris , J Dunne Mark , Salomon-Estebanez Maria , Harper Simon , W Nutter Paul , Dastamani Antonia , Senniappan Senthil , Banerjee Indraneel

Background and Objective: Continuous Glucose Monitoring (CGM) is gaining in popularity for patients with paediatric hypoglycaemia disorders such as Congenital Hyperinsulinism (CHI), but no standard measures of accuracy or associated clinical risk are available. A small number of studies have shown suboptimal accuracy of CGM in CHI but assessments have been inconsistent, incomplete and offer no measure of clinical application. Error grids that categorise clinic...